Pertumbuhan in vitro stek mikro singkong (Manihot esculenta Crantz.)

Ardian Ardian

Sari


Propagation of cassava through in vitro culture is needed by farmers and agro industries to fulfill the need of the best and the newest clone as soon as after that clone is released by government. The objective of this research is to know the effect of the application of some concentration sucrose and activated charcoal on in vitro and ex vitro growth of micro-cutting of cassava. Explants used were one-node cuttings of cassava, which were derived from sterile shoot culture. The explants were cultured on MS basal medium supplemented with 0,2 mg.l-1 benzyl adenine. This research was arranged in completely randomized design with the treatments
consisting of some concentrations of sucrose: 3%, 4% and 5% and concentration of activated charcoal: 0% and 0,1%. Each treatment was replicated 7 times with 4 explants in each experiment unit. The best growth micro-cutting of cassava in vitrowas achieved by treatment of 3% sucrose without activated charcoal.

Keywords: Micro-cutting, Cassava


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Jurnal Penelitian Pertanian Terapan

Volume 10, Nomor 3, September 2010

Fridborg, G., M. Pedersen, L.E. Landstrom and T. Eriksson. 1978. The effect of activated charcoal

on tissue cultures: adsorption ofmetabolites inhibiting morphogenesis. Physiol. Plant. 43:

-106.

George, F.E. and P.D. Sherington. 1984. Plant Propagation by Tissue Culture. Exegetics Ltd.

England.

Hdider, C. and Y. Desjardius. 1994. Effect of sucrose on photosynthesis and phosphoenolpyruvat

carboxylase activity of in vitro cultured strawberry planlets. Plant Cell, Tissue and Organ

Culture 36: 27-33.

Nhut, D.T., B.V. Le, S. Fukai, M. tanaka and T.T. Van. 2001. Effects of activated charcoal, explant

size, explant position and sucrose concentration on plant and shoot regeneration of Lilium

longiflorum via young stem culture. Plant Growth Reg. 33: 59-65.

Peck, D.E. and B.G. Cumming. 1986. Beneficial effects of activated charcoal on bublet production in

tissue cultures of Muscari armeniacum. Plant Cell Tissue Organ Cult. 6: 9-14.

Pierik, R.L.M. 1987. In Vitro Culture of Higher Plants. Martin Nijhoff Publ. Netherland. p. 344.

Schnapp, S.R. and J.E. Preece. 1986. In vitro growth reduction of tomato and carnation microplants.

Plant Cell Tissue Organ Cult. 6: 3-8.

Sha Valli Khan, P.S., J.F. Hausman and K.R. Rao. 1999. Effect of agar, MS medium strength,

sucrose and polyamines on in vitro rooting of Syzygium alternifolium. Biol.Plant. 42(3): 333-

Shim, S.W., E.J. Hahn and K.Y. Paek. 2003. In vitro and ex vitro growth of gravevine rootstock

`5BB` as influenced by number of air exchanges and the presence or absence of sucrose in

culture media. Plant Cell Tissue Organ Cult. 75: 57-62.

Thorpe, T.A. 1982. Carbohydrate utilization and metabolism. In J.M. Bonga and D.J. Durzan (eds).

Tissue culture in forestry. Martinus Nijhoff. London.

Weatherhead, M.A., J. Burdon and G.G. Henshaw. 1978. Some effect of activated charcoal as an

additive to plant tissue culture media. Z.Pflanzenphysiol. 89: 141-147.

Weatherhead, M.A., J. Burdon and G.G. Henshaw. 1979. Effect of activated charcoal as an additive

to plant tissue culture media: part 2. Z.Pflanzenphysiol. 94: 399-405.Ardian. 2008. Pertumbuhan in vitro dan ex vitro tunas mikro tanaman nilam Aceh (Pogostemon

cablin Benth.) dengan perlakuan berbagai konsentrasi sukrosa dan arang aktif. J.Agrista: 36-

Badan pusat Statistik Lampung. 2008. Lampung Dalam Angka 2006. BPS Lampung dan Bappeda

Propinsi Lampung. 622 hlm.

Balai Informasi Pertanian Irian Jaya. 1995. Budidaya Singkong (Manihot esculenta Cranzt.).

Lembar Informasi Pertanian, BIP Irian Jaya 150/95. http://www.pustaka-deptan.go.id.

Custodio, L., M.A.M. Loucao and A. Romano. 2004. Influence of sugars on in vitro rooting and

acclimatization of carob tree. Biol.Plant. 48(3): 469-472.

Esti dan K. Prihatman. 2000. Tepung tapioca. http://bebas.vlsm.org/v12/artikel/pangan.

Food and Agriculture Organization. 2007. Cassava. http://www.fao.org.




DOI: http://dx.doi.org/10.25181/jppt.v10i3.259

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