Pengaruh Berbagai Konsentrasi Benzil Adenin dan Asam Naftalen Asetat Pada Kultur In vitro Singkong (Manihot esculenta Crantz.)
DOI:
https://doi.org/10.25181/jppt.v12i1.197Abstract
Propagation of cassava through in vitro culture is needed by farmers and agro industries to fulfill the need of the best and the newest clone as soon as after it is released by government. The objective of this research was to know the effects of the application of some concentrations of benzyl adenine and naphthalene acetic acid on in vitro growth and multiplication of micro-shoot of cassava. Explants used were one-node green cuttings of cassava, which were derived from cutting seedling. This research was arranged in completely randomized design with the treatments consisting of some concentrations of benzyl adenine 0.2, 0.4, and 0.8 mg/l and concentrations of naphthalene acetic acid: 0 and 0.1 mg/l. Each treatment was replicated 10 times with 2 explants in each experiment unit. The best growth and multiplication shoot of cassava in vitro was achieved by treatment of 0,2 mg/l benzyl adenine with 0,1 mg/l naphthalene acetic acid. Keywords: Benzyl Adenine, Naphthalene Acetic Acid, CassavaDownloads
References
Ardian, Kresna
dan Agustiansyah: Pengaruh Berbagai Konsentrasi Benzil Adenin...
Volume 12, Nomor 1, Januari 2012 49
Badan pusat Statistik Lampung. 2008. Lampung Dalam Angka 2006. BPS Lampung dan
Bappeda Propinsi Lampung. 622 hlm.
Balai Informasi Pertanian Irian Jaya. 1995. Budidaya Singkong (Manihot esculenta Cranzt.).
Lembar Informasi Pertanian, BIP Irian Jaya 150/95. http://www.pustaka-deptan.go.id.
Cristea, V., A.T. Brummer, L. Jarda and M. Miclaus. 2010. In vitro culture initiation and
phytohormonal influence on Dianthus henteri- a Romanian endemic species.
Rom.Biotech. Lett. 15(1): 25-33.
George, E.F. 1996. Plant propagation by tissue culture In Practice. 2nd
edition. Exegetics.
England.
Hossain, S.N., M.K. Munshi, M.R. Islam, L. Hakim and M. Hossain. 2003. In vitro propagation of
plum (Zyziphus jujube Lam.). Plant Tissue Cult. 13(1): 81-84.
Khalafalla, M.M., E.I. Elgaali and M.M. Ahmed. 2007. In vitro multiple shoot regeneration from
nodal explants of Vernonia amygdalina- an important medicinal plant. Af.Crop Sci. Conf.
Proc. Vol 8: 747-752.
Khaleghi, A., A. Khalighi, A. Sahraroo, M. Karimi, A. Rasoulnia, I.N. Ghafooni and R. Ataei.
In vitro propagation of Alstroemeria cv. `Fuego`. Am-Euras J.Agric & Environ.
Sci. 3(3): 492-497.
Khosravi, P., M.J. Kermani, G.A. Nematzadeh and M.R. Bihamta. 2007. A protocol for mass
production of rosa hybrid cv. Iceberg through in vitro propagation. Iran J. Biotech
(2):100-104.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco
tissue culture. Physiol. Plant. 15: 473-497.
Naz, S., S. Illyas, S. Javad and A.Ali. 2009. In vitro clonal multiplication and acclimatization of
different varieties of turmeric (Curcuma longa L.) Pak. J. Bot. 41(6): 2807-2816.
Oliveira, M.L.P., M.G.C. Costa, C.V. Silva and W.C. Otoni. 2010. Growth regulators, culture
media, and antibiotics in the in vitro shoot regeneration from mature tissue of citrus
cultivars. Pesq. Agropec.Bras. 45(7): 654-660.
Pant, B. and S. Manandhar. 2007. In vitro propagation of carrot (Daucus carota) L. Sci. World
(5): 51-53.
Rani, S. and J.S. Rana. 2010. In vitro propagation of Tylophora indica- influence of explanting
season, growth regulator synergy, culture passage and planting, substrate. J Amm. Sci.
(2): 385-392.Al Malki, A.A.H.S. and K.M.S. Elmeer. 2010. Influence of auxin and cytokinine on in vitro
multiplication of Ficus Anastasia. Af. J. Biotech. 9(5):635-639.
Ardian dan Yuliadi, E. 2009. Pertumbuhan dan perbanyakan tunas mikro singkong (Manihot
esculenta Crantz.) secara in vitro pada berbagai konsentrasi benzil adenin. J. Agrotropika
(1): 19-22.
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