Pengaruh Berbagai Konsentrasi Benzil Adenin dan Asam Naftalen Asetat Pada Kultur In vitro Singkong (Manihot esculenta Crantz.)

Authors

  • Ardian Ardian Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Lampun
  • Kresna Kresna Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Lampung
  • Agustiansyah Agustiansyah Jurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Lampun

DOI:

https://doi.org/10.25181/jppt.v12i1.197

Abstract

Propagation of cassava through in vitro culture is needed by farmers and agro industries to fulfill the need of the best and the newest clone as soon as after it is released by government. The objective of this research was to know the effects of the application of some concentrations of benzyl adenine and naphthalene acetic acid on in vitro growth and multiplication of micro-shoot of cassava. Explants used were one-node green cuttings of cassava, which were derived from cutting seedling. This research was arranged in completely randomized design with the treatments consisting of some concentrations of benzyl adenine 0.2, 0.4, and 0.8 mg/l and concentrations of naphthalene acetic acid: 0 and 0.1 mg/l. Each treatment was replicated 10 times with 2 explants in each experiment unit. The best growth and multiplication shoot of cassava in vitro was achieved by treatment of 0,2 mg/l benzyl adenine with 0,1 mg/l naphthalene acetic acid. Keywords: Benzyl Adenine, Naphthalene Acetic Acid, Cassava

Downloads

Download data is not yet available.

References

Ardian, Kresna

dan Agustiansyah: Pengaruh Berbagai Konsentrasi Benzil Adenin...

Volume 12, Nomor 1, Januari 2012 49

Badan pusat Statistik Lampung. 2008. Lampung Dalam Angka 2006. BPS Lampung dan

Bappeda Propinsi Lampung. 622 hlm.

Balai Informasi Pertanian Irian Jaya. 1995. Budidaya Singkong (Manihot esculenta Cranzt.).

Lembar Informasi Pertanian, BIP Irian Jaya 150/95. http://www.pustaka-deptan.go.id.

Cristea, V., A.T. Brummer, L. Jarda and M. Miclaus. 2010. In vitro culture initiation and

phytohormonal influence on Dianthus henteri- a Romanian endemic species.

Rom.Biotech. Lett. 15(1): 25-33.

George, E.F. 1996. Plant propagation by tissue culture In Practice. 2nd

edition. Exegetics.

England.

Hossain, S.N., M.K. Munshi, M.R. Islam, L. Hakim and M. Hossain. 2003. In vitro propagation of

plum (Zyziphus jujube Lam.). Plant Tissue Cult. 13(1): 81-84.

Khalafalla, M.M., E.I. Elgaali and M.M. Ahmed. 2007. In vitro multiple shoot regeneration from

nodal explants of Vernonia amygdalina- an important medicinal plant. Af.Crop Sci. Conf.

Proc. Vol 8: 747-752.

Khaleghi, A., A. Khalighi, A. Sahraroo, M. Karimi, A. Rasoulnia, I.N. Ghafooni and R. Ataei.

In vitro propagation of Alstroemeria cv. `Fuego`. Am-Euras J.Agric & Environ.

Sci. 3(3): 492-497.

Khosravi, P., M.J. Kermani, G.A. Nematzadeh and M.R. Bihamta. 2007. A protocol for mass

production of rosa hybrid cv. Iceberg through in vitro propagation. Iran J. Biotech

(2):100-104.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco

tissue culture. Physiol. Plant. 15: 473-497.

Naz, S., S. Illyas, S. Javad and A.Ali. 2009. In vitro clonal multiplication and acclimatization of

different varieties of turmeric (Curcuma longa L.) Pak. J. Bot. 41(6): 2807-2816.

Oliveira, M.L.P., M.G.C. Costa, C.V. Silva and W.C. Otoni. 2010. Growth regulators, culture

media, and antibiotics in the in vitro shoot regeneration from mature tissue of citrus

cultivars. Pesq. Agropec.Bras. 45(7): 654-660.

Pant, B. and S. Manandhar. 2007. In vitro propagation of carrot (Daucus carota) L. Sci. World

(5): 51-53.

Rani, S. and J.S. Rana. 2010. In vitro propagation of Tylophora indica- influence of explanting

season, growth regulator synergy, culture passage and planting, substrate. J Amm. Sci.

(2): 385-392.Al Malki, A.A.H.S. and K.M.S. Elmeer. 2010. Influence of auxin and cytokinine on in vitro

multiplication of Ficus Anastasia. Af. J. Biotech. 9(5):635-639.

Ardian dan Yuliadi, E. 2009. Pertumbuhan dan perbanyakan tunas mikro singkong (Manihot

esculenta Crantz.) secara in vitro pada berbagai konsentrasi benzil adenin. J. Agrotropika

(1): 19-22.

Published

2017-07-13

How to Cite

Ardian, A., Kresna, K., & Agustiansyah, A. (2017). Pengaruh Berbagai Konsentrasi Benzil Adenin dan Asam Naftalen Asetat Pada Kultur In vitro Singkong (Manihot esculenta Crantz.). Jurnal Penelitian Pertanian Terapan, 12(1). https://doi.org/10.25181/jppt.v12i1.197

Issue

Section

Artikel

Most read articles by the same author(s)