Respons Planlet Stevia (Stevia rebaudiana) terhadap Penambahan berbagai Konsentrasi Thidiazuron (TDZ) dan Naphthalene Acetic Acid (NAA) secara in Vitro

Muhammad Dimas; Wiwik Indrawati; Dedi Supriyatdi

doi:10.25181/jaip.v11i2.2849

Authors

Muhammad Dimas Politeknik Negeri Lampung
Wiwik Indrawati Politeknik Negeri Lampung
Dedi Supriyatdi Politeknik Negeri Lampung

DOI:

https://doi.org/10.25181/jaip.v11i2.2849

Keywords:

concentration, in vitro, NAA, stevia, TDZ

Abstract

Stevia can be developed by vegetative and generative propagation, while the generative propagation of stevia can be done through seeds. Conventional provision of seeds or seedlings has a low success rate. Propagation via tissue culture is faster, produces more seeds, and is identical to the parent, so the plants’ quality is expected. Plant growth regulator (PGR) often used for tissue culture is type auxins, cytokinins and gibberellins. The function of auxin and cytokinin for division cells, cell elongation, cell differentiation and organ formation. This research aims to get the concentration as well as the best combination for the growth of stevia plants against the administration of thidiazuron (TDZ) and naphthalene acetic acid (NAA). Research carried out by the randomised block design (RBD) factorial pattern consisting of two factors: TDZ and NAA. The first factor is TDZ concentration consisting of three levels: 0 mg.l-1 (T0), 1 mg.l-1 (T1), and 2 mg. l-1 (T2). The second factor is NAA concentration, which consists of three levels: 0 mg.l-1 (N0), 1 mg.l-1 (N1), and 2 mg.l-1 (N1). If there is a difference in data analysis using the F test, then proceed with the BNT level test of 5%. Based on the research results obtained, the influence of TDZ growth regulators and no effect of the concentration of NAA growth regulator on stevia bud multiplication book number. There is an effect of TDZ on plantlet height, leaves, and stevia shoot multiplication nodes.

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References

Abbas, B. (2011). Prinsip Dasar Teknik Kultur Jaringan. Alfabeta.

Asmono, S. L. (2020). In vitro regeneration of Stevia rebaudiana Bertoni from internode and leaf explants using different concentrations of BAP (6-Benzyl Amino Purine). In IOP Conference Series: Earth and Environmental Science (Vol. 411, No. 1, p. 012004). IOP Publishing.

Asmono, S. L., Sari, V. K., & Wardana, R. (2018). Respons pertumbuhan tunas mikro stevia (Stevia rebaudiana Bertoni) secara in vitro pada beberapa jenis sitokinin dan konsentrasi air kelapa. Agrin, 21(2), 146-154.

Dewanti, P. (2018). Teknik Kultur Jaringan Tanaman: Prinsip Umum dan Metode Aplikasi di Bidang Bioteknologi Pertanian. Universitas Jember.

Dinarti, D., Sayekti, U., & Alitalia, Y. (2010). Kultur Jaringan Kantong Semar (Nepenthes mirabilis). Jurnal Hortikultura Indonesia, 1(2), 59-65.

Habibah, N. A., Rahayu, E. S., & Anggraito, Y. U. (2021). Buku Ajar Kultur Jaringan Tumbuhan. Deepublish.

Hadiyana, A., & Syabana, M. A. (2015). Iniasi tunas secara kultur jaringan pada stevia (Stevia rebaudiana Bertoni) dengan kosentrasi indole butyric acid (IBA) and benzyl amino purine (BAP) yang berbeda. Jurnal Agroekoteknologi, 7(2), 147-152.

Harahap, R. A. (2018). Pengaruh Pemberian NAA (Naphtalene Aceitc Acid) dan TDZ (Thiadiazurone) terhadap Pertumbuhan Planlet Tanaman Kentang (Solanum tuberosum L.) [Unpublished undergraduate thesis, Univeritas Muhammadiyah Sumatera Utara].

Immalasari, R. N. (2018). Variasi Media dan Konsentrasi Zat Pengatur Tumbuh TDZ pada Induksi Kalus Pucuk Jati (Tectona grandis) secara In Vitro [Unpublished undergraduate thesis, Universitas Hasanuddin].

Lestari, E. 2010. Peranan zat pengatur tumbuh dalam perbanyakan tanaman melalui kultur jaringan. Jurnal Agrobioteknologi, 7(1), 63-68.

Limanto, A. (2017). Stevia, pemanis pengganti gula dari tanaman Stevia rebaudiana. Jurnal Kedokteran Meditek, 23(61), 1-12. https://doi.org/10.36452/jkdoktmeditek.v23i61.1466

Madhusudhanan, K., & Rahiman, B. A. (2000). The effect of activated charcoal supplemented media to browning of in vitro cultures of Piper species. Biologia Plantarum, 43(2), 297-299.

Mahadi, I., Syafi’i, W., & Agustiani, S. (2015). Kultur jaringan jeruk kasturi (Citrus microcarpa) dengan menggunakan hormon kinetin dan naftalen acetyl acid (NAA). Dinamika Pertanian, 30(1), 37-44.

Manullang, A. 2019. Pengaruh Pemberian Jenis Sitokinin dan Auksin terhadap Pembentukan Tunas Mikro Lili Putih (Lilium longiflorum Thunb. var. Liani) [Unpublished undergraduate thesis, Universitas HKBP Nommensen].

Mirah, T., Undang, U., Sunarya, Y., & Ermayanti, T. M. (2021). Pengaruh konsentrasi sitokinin dan jenis media terhadap pertumbuhan eksplan buku stevia (Stevia rebaudiana Bert.) Tetraploid. Media Pertanian, 6(1), 1-11

Mendrofa, F. N. E., Saputro, N. W., & Afifah, L. (2021). Induction shoots from callus of cucumber apple (Cucumis sp.) using a combination of benzil amino purine and naphtalene acetic acid concentrations in vitro. Jurnal Mangifera Edu, 5(2), 103-120.

Ni'mah, A. (2018). Multiplikasi Tunas Stevia (Stevia rebaudiana) pada Berbagai Macam Media Dasar dan Konsentrasi 6-Benzyl Amino Purin (BAP) secara In Vitro [Unpublished undergraduate thesis, Universitas Islam Negeri Malik Ibrahim].

Pamungkas, S. S. T., & Nopiyanto, R. (2020). Pengaruh zat pengatur tumbuh alami dari ekstrak tauge terhadap pertumbuhan pembibitan budchip tebu (Saccharum officinarum L.) varietas Bululawang. Mediagro, 16(1), 68-80.

Rahman, N., Fitriani, H., Rahman, N., & Hartati, N. S. (2021). Pengaruh beragam zat pengatur tumbuh terhadap induksi kalus organogenik dari ubi kayu (Manihot esculenta Crantz) Genotipe Gajah dan Kuning. Jurnal Ilmu Dasar, 22(2), 119-126.

Restiani, R., Semiarti, E., & Indrianto, A. (2016). Konservasi anggrek hitam (Coelogyne pandurata Lindl.) melalui mikropropagasi pada berbagai medium kultur. Prosiding Simposium Nasional Pendidikan Biologi (Symbion 2016), 27, 393-404.

Sakinah, N. A. (2017). Pengaruh Kombinasi 2, 4 D dan Thidiazuron (TDZ) terhadap Pertumbuhan Kalus Daun Legundi (Vitex trifolia Linn) pada Media MS [Unpublished undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim].