Multiplikasi Mata Tunas Pisang ‘Cavendish’ In Vitro Pada Berbagai Konsentrasi Benziladenin
DOI:
https://doi.org/10.25181/jppt.v18i1.748Keywords:
bananas, BA, in vitro, shoot multiplicationAbstract
Banana is one of the most important global agriculture commodities. Asia accounts for the production of bananas amounted to 56.4% of total banana in the world and Indonesia is one of the banana-producing countries in Asia. The demand for bananas increased need is anticipated with good cultivation techniques to meet domestic and international market demand. Plant regeneration in vitro tissue culture techniques or can be used to produce propagules true-to-type in large quantities in a relatively short period and free from disease. This research aims to study the influence of various concentrations of BA (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg/l) against the growth and reproduction of Cavendish banana propagules in vitro. Time appears buds, propagules length and the number of propagules per explant, and the number of primary roots of plants per explant observed after eight weeks of culture. The experiment results show that after explant grown for eight weeks on the media's treatment shows the different response. The increased concentration of BA can increase the number of shoots. The increased concentration of BA from 0.5 mg/l be 1-3 mg/l increased the number of propagules, but the number of propagules will decrease in the concentration of BA more 3.5 or 4 mg/l. In addition to BA concentration 0.5, 1 and 1.5 mg/l MS media into producing shoot buds appear faster time high, highest propagules and root amount more on banana propagules Cavendish, but the number of shoots is fewer. The best of BA concentrations to induce propagules plant bananas Cavendish is 3.0 mg/l.Downloads
References
Avivi, S., Ikrarwati, I., 2004. Mikropropagasi pisang Abaca (Musa textilis Nee) melalui teknik kultur jaringan. Ilmu Pertan. 11, 17–34.
BPS, 2014. Tabel Dinamis [WWW Document]. URL http://www.bps.go.id/site/pilihdata (accessed 1.25.18).
Ferdous, M.H., Billah, A.A.M., Mehraj, H., Taufique, T., Uddin, A.F.M.J., 2015. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. J. Biosci. Agric. Res. 3, 87–95.
Gahan, P.B., George, E.F., 2008. Adventitious Regeneration, in: George, E.F., Hall, M.A., Klerk, G.-J. De (Eds.), Plant Propagation by Tissue Culture Volume 1. The Background. Springer, The Netherlands, pp. 355–401.
Hapsoro, D., Yusnita, 2016. Kultur Jaringan Untuk Perbanyakan Klonal Kelapa Sawit (Elaeis guineesis Jacq.). CV. Anugrah Utama Raharja (AURA), Bandar Lampung.
Kalimuthu, K., Saravanakumar, M., Senthilkumar, R., 2007. In Vitro Micropropagation of Musa sapientum L. (Cavendish Dwarf). African J. Biotechnol. 6, 1106–1109.
Muhammad, A., Rashid, H., Hussain, I., 2007. Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai.’ HORTSCIENCE 42, 1253–1255.
Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Sari, E.P., 2012. Multiplikasi Tunas Pisang Ambon Kuning Sebagai Respons Terhadap Konsentrasi Benzyladenine Dan Indole-3-Acetic Acid. Universitas Lampung.
Sipen, P., Davey, M.R., 2012. Effects of N6-benzylaminopurine and indoleacetic acid on in vitro shoot multiplication, nodule-like meristem proliferation and plant regeneration of Malaysian bananas. Trop Life Sci Res 23, 67–80.
Staden, J. Van, Zazimalova, E., George, E.F., 2008. Plant Growth Regulators II: Cytokinins, their Analogues and Antagonists, in: George, E.F., Hall, M.A., Klerk, G.-J. De (Eds.), Plant Propagation By Tissue Culture 3rd Edition. Springer, The Netherlands, pp. 205–226.
Taji, A., Kumar, P.P., Lakshmanan, P., 2002. In vitro plant breeding. Food Products Press, New York.
Yusnita, 2003. Kultur Jaringan: Cara Memperbanyak Tanaman secara Efisien. AgroMedia Pustaka, Jakarta.
Yusnita, Y., Danial, E., Hapsoro, D., 2015. In Vitro Shoot Regeneration of Indonesian Bananas (Musa spp.) cv. Ambon Kuning and Raja Bulu, Plantlet Acclimatizationand Field Performance. Agrivita 37, 51–58. https://doi.org/DOI: http://doi.org/10.17503/agrivita.v37i1.438
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.