Kultur Embrio Kelapa Kopyor Menggunakan Beberapa Konsentrasi BA dan Air Kelapa
DOI:
https://doi.org/10.25181/jppt.v20i3.1929Keywords:
Coconut kopyor, embryo culture, BA, coconut waterAbstract
Coconut Kopyor is an abnormal fruit, the ordinary coconut flesh is attached to the shell and separated from coconut water while Coconut Kopyor, fruit flesh is not attached to the shell but is mixed with coconut water. Kopyor coconut cannot be used as a seed (seed). The growth of kopyor coconut embryos can only be done in a laboratory with embryo culture technology. Embryo culture is the only way to produce true to type coconut kopyor seeds that can produce 80% kopyor coconut in one kopyor coconut plant, kopyor coconut seedlings produced can support government programs in increasing the productivity of kopyor coconut through the provision of quality seeds. Research on kopyor coconut embryo culture was carried out at the Laboratory of Tissue Culture at Lampung State Polytechnic. The experiment was carried out using a RAL, with 15 treatment combinations of BA media formulations (0, 2, and 4) with coconut water (0 ml / l, 100, 150, 200, 250 ml / l). The observed variables were the percentage of embryos sprouting, when the shoot appeared, shoot height, number of roots, and number of leaves. The results showed that the use of BA 4 mg / l without the addition of coconut water increased the percentage of sprouts, faster time for shoots, and highest shoot height.Downloads
References
Burun, B. & P. E. C. (2002) ‘Embryo culture in barley (Hordeum vulgare L.)’, Turkish Journal of Biology, (26), pp. 175–180.
Jimenes, V.M., J. Castillo, E. Tavares, E. Guevara, and M. M. (2006) ‘In vitro propagation of the neotropical giant bamboo (Guadua angustifolia Kunth) through axillary shoot proliferation’, Tissue and Organ Culture Journal, (86), pp. 389–395.
Laukkanen, H., H. Haggman, S. Kontunen-Soppela, A. H. (1999) ‘Tissue browning of in vitro cultures of Scots pine: Role of peroxidase and polyphenol oxidase’, Physiol. Plant, (106), pp. 337–343.
Mahmud, Z. (2000) Petunjuk teknis budidaya kelapa kopyor. Departemen Kehutanan dan Perkebunan. Dirjen Perkebunan. Jakarta da. Jakarta: Dirjen Perkebunan.
Mashud, N. (2010) Pengembangan metode kultur embryo kelapa kopyor yang lebih efisien (30 %). Manado: Balai Penelitian Tanaman Kelapa dan Palma Lain.
Novarianto, H. dan M. (2000) ‘Koleksi dan konservasi jenis-jenis kelapa unik’, Makalah poster dalam Simposium Pengelolalan Plasma nutfah dan Pemuliaan.
Osterac, G., M.Z. Frass, T. Vodenik, and Z. L. (2005) ‘The propagation of chesnut (Cactanea sativa Mill) Nodal Explants’, Acta Agri. Slovenica Journal, (85), pp. 411–418.
Raghavan, V. (2003) ‘One hundred years of zygotic embryo culture investigations. In Vitro’, Celuler and Development Biology - Plant, (39), pp. 437–442.
Roostika, I., N. Sunarlim, dan I. M. (2005) ‘Mikropropagasi tanaman manggis (Garcinia mangostana)’, Jurnal Agrobiogen, (J. Agrobiogen), pp. 20–25.
Rufaida, A., Waeniaty, M. dan I. N. S. (2013) ‘Organogenesis tanaman bawang merah (Alium ascalonicum, L) lokal Palu secara in vitro pada medium MS dengan penambahan IAA dan BAP’, Online Jurnal of Natural Science, 2(2), pp. 1–7.
Samunte, J. L., E. M. T. Mendoza, L. L. Ilag, MN. B. De la Cruz, dan D. A. R. (1989) ‘Galactomannan degrading enzymes in maturing normal and makapuno and germinating normal coconut endosprem’, Phytochemistry, (8), pp. 2269–2273.
Sudarsono, I. Maskromo, D. Dinarti, MS. Rahayu, D. Sukma, Yuliasti, M.LA. Hosang, dan H. N. (2013) ‘Status penelitian dan pengembangan kelapa kopyor di Indonesia.’, Prosiding Konferensi Nasional Kelapa VIII.
Sukendah, Sudarsono, Witjaksono. & Khumaida, N. (2008) ‘Perbaikan teknik kultur embrio kelapa kopyor (Cocos nucifera L.) asal Sumenep Jawa Timur melalui penambahan bahan aditif dan pengujian periode subkultur’, Buletin Agronomi., 36(1), pp. 16–23.
Sukendah (2009) Teknologi pembiakan kultur in vitro dan analisis molekuler pada tanaman kelapa kopyor. Institut Pertanian Bogor.
Triningsih, A. Luthfi, M. S. dan L. A. P. P. (2013) ‘Pertumbuhan eksplan puar tenangau (Elletariopsis sp) secara in vitro’, Jurnal Online Agroteknologi, 1(2), pp. 276–285.
Yuniastuti, E., Praswanto, Harminingsih, I. (2010) . ‘Pengaruh Konsentrasi BAP Terhadap Multiplikasi Tunas Anthurium (Anthurium Andraeanum Linden) Pada Beberapa Media Dasar Secara In Vitro’, Caraka Tani, (XXV), p. No.1.
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