Uji Daya Hambat Isolat Bakteri Asam Laktat Usus Itik (Anas Domestica) Pada Bakteri Gram Positif Dan Pola Pertumbuhan Isolat Bakteri Usus Itik Pada Media Mrs Broth

Authors

  • Rudy Sutrisna Jurusan Peternakan Fakultas Pertanian Unila
  • Nugroho Ekowati Jurusan Biologi Fakultas MIPA Unila
  • Diah Rahmawati Jurusan Biologi Fakultas MIPA Unila

DOI:

https://doi.org/10.25181/jppt.v13i1.168

Abstract

At this time unknown bacterial isolates inhibit the ability of the isolated intestine of ducks against Gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus. In addition, the growth pattern is also unknown bacterial isolates grew on the medium MRS Broth. This study aims to determine the inhibition of intestinal bacterial isolates antibacterial extract of duck against Gram-positive bacteria and determine the pattern of growth of intestinal bacteria duck isolates were selected on MRS Broth media. Determination of the inhibition assay duck intestine bacterial isolates using the wells, while for the determination of growth patterns of duck intestine bacterial isolates by counting directly the number of bacterial cells by microscopy. The results showed that the bacterial isolates capable of inhibiting the growth of colon duck Gram-positive bacteria. Isolates that produce the greatest diameter of the results of this research that isolates B4 and B9 at 20.2 mm and 17.9 mm long production time 4 days. Growth of intestinal bacteria isolates duck B4 shows the phase lag for 2 hours and logarithmic phase for 17 hours, while isobat B9 shows the phase lag for 3 hours and logarithmic phase for 10 hours. Keywords: Intestinal Bacterial Isolates Ducks, Inhibition Power Test, Growth Patterns, Staphylococcus aureus, and Bacillus subtilis

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References

Rudy Sutrisna dan Nugroho Ekowati: Uji Daya Hambat Isolat Bakteri Asam Laktat Usus Itik…

Volume 13, Nomor 1, Januari 2013 59

Napitupulu, N., T. Yulinery, dan R. Hardiningsih. 2000. Pengaruh Lama Penyimpanan, Suhu dan

Media terhadap Kemampuan Antibakteri yang Dihasilkan Lactobacillus dalam Menghambat

pertumbuhan Beberapa Bakteri Patogen. Bogor: Proyek penelitian Pengembangan dan

Pendayagunaan Biota Darat, Pusat Penelitian Biologi LIPI.

Nester. Eugene W, Anderson. Dennis G, Roberts. C evans Jr, Nester, Martha T. 2009. Microbiology a

Human Perspective 6th

Edition. McGraw-Hill : New York

Nissen-Meyer, J., Holo, H., HÃ¥varstein, L. S., Sletten, K. & Nes, I. F. 1992. A novel lactococcal

bacteriocin whose activity depends on the complementary action of two peptides. J

Bacteriol 174, 5686–5692.

Sutrisna, R., S. Nurjanah. 2010. Isolasi non-starch Polysacharides sebagai Prebiotik dan Bakteri

sebagai Probotik dalam Sistem Pencernaan Itik. Laporan Penelitian Hibah Bersaing Tahun

Fakultas Pertanian Universitas Lampung Bandar Lampung

Young, J. 1998. European market developments in prebiotic- and probiotic-containing foodstuffs.

British J. Nutr. 80. Suppl. 2, S231-S233.Abun. 2008. Hubungan Mikroflora Dengan Metabolisme Dalam Saluran Pencernaan Unggas Dan

Monogastrik. Universitas Padjadjaran : Jatinangor

Anggorodi, R. 1995. Nutrisi Aneka Ternak Unggas. Gramedia Pustaka Utama: Jakarta

Daeschel, M., A. 1989. Antimicrobial substance from lactic acid bacteria for use as food

preservation. J Food Technol 43: 148-155.

deMan, JC, M. Rogosa, and ME Sharpe. 1960. A medium for the cultivation of lactobacilli. J.

Bacteriol:. 23 130

Fuller R. 1991. ProbioticTheScientificBasis. Chapmanand Hall : London

Hasim. 2003. Menanam Rumput, Memanen Antibiotik. Jakarta :Kompas No. 127. Tahun ke-39

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Published

2017-07-05

How to Cite

Sutrisna, R., Ekowati, N., & Rahmawati, D. (2017). Uji Daya Hambat Isolat Bakteri Asam Laktat Usus Itik (Anas Domestica) Pada Bakteri Gram Positif Dan Pola Pertumbuhan Isolat Bakteri Usus Itik Pada Media Mrs Broth. Jurnal Penelitian Pertanian Terapan, 13(1). https://doi.org/10.25181/jppt.v13i1.168

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Vol. 13 No. 1 (2013)

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Artikel

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