Isolasi dan Pertumbuhan Miselium Jamur Grigit (Schizophyllum Commune) Pada Berbagai Media Biakan Murni

Yeni Yeni; Nanang Wahyu  Prajaka; Fahri  Ali; Abied  Khafidhan; Ratih  Rahhutami; Raissa Tazkia  Nurhaliza; Desty Aulia  Putranti

doi:10.25181/jppt.v26i1.4802

Authors

Yeni Yeni Politeknik Negeri Lampung
Nanang Wahyu Prajaka 1Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan
Fahri Ali Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan, Politeknik Negeri Lampung
Abied Khafidhan Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan, Politeknik Negeri Lampung
Ratih Rahhutami Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan, Politeknik Negeri Lampung
Raissa Tazkia Nurhaliza Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan, Politeknik Negeri Lampung
Desty Aulia Putranti Program Studi Teknologi Produksi Tanaman Hortikultura, Jurusan Budidaya Tanaman Pangan, Politeknik Negeri Lampung

DOI:

https://doi.org/10.25181/jppt.v26i1.4802

Abstract

The split gill mushroom (Schizophyllum commune) is an edible fungus that can be readily identified by its distinctive morphology and has considerable potential for development as a food source and a producer of bioactive compounds in Indonesia. Consequently, the establishment of an efficient propagation method through pure culture is essential. This study aimed to isolate S. commune and to evaluate its mycelial growth on different pure culture media. Isolation was conducted using a tissue culture technique from healthy and fresh fruiting bodies. The culture media evaluated were Potato Dextrose Agar (PDA), rice washing water medium, coconut water medium, rice bran medium, and corn boiling water medium. Mycelial growth rate, radial growth diameter, and mycelial morphological characteristics were used as growth parameters. The results indicated that the type of culture medium significantly influenced the mycelial growth of S. commune. PDA, rice washing water, and rice bran media supported the highest and most uniform mycelial growth. Mycelia grown on PDA and rice bran media exhibited a whitish-yellow coloration, dense and uniform growth, and a cotton-like texture. These findings suggest that both conventional and alternative media derived from locally available resources can effectively support the pure culture of S. commune, offering potential for more cost-effective and sustainable cultivation practices.

Downloads

Download data is not yet available.

References

Albina, T., Kaur, J. and Bhadariya, V. (2023). Schizophyllum commune: A Promising Functional Ingredient for Food and Medicine. Journal of Food Chemistry and Nanotechnology, 9(1), pp. 204–210. Available at: https://doi.org/10.17756/jfcn.2023-s1-026.

Alfianti, F., Cahya Murti, A. and Burhanuddin Adenan, M. (2021). Pasteurization of Coconut Water and Rice washing water as A Suplement for The Life of Oyster Mushroom Cultivation Media. Agritech, 23(1), pp. 24–31. Available at: https://www.ncbi.nlm.nih.gov/.

Carrasco, J. Zied, D. C., Pardo, J. E., Preston, G. M., Gimenez, A. P. (2018). Supplementation in Mushroom Crops and Its Impact on Yield and Quality. AMB Express, 8(1), pp. 1–10. Available at: https://doi.org/10.1186/s13568-018-0678-0.

Chang, S. T., & Miles, P. G. 2004. Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact (2nd ed.). Boca Raton: CRC Press.

Chen, Z. Yin, C., Fan, X., Ma, K., Yao, F., Zhou, R., Shi, D., Cheng, W., Gao, H. (2020). Characterization of Physicochemical and Biological Properties of Schizophyllum Commune Polysaccharide Extracted With Different Methods. International Journal of Biological Macromolecules, 156, pp. 1425–1434. Available at: https://doi.org/10.1016/j.ijbiomac.2019.11.183.

Dasanayaka, P.N. and Wijeyaratne, S.C. (2017). Cultivation of Schizophyllum commune Mushroom on Different Wood Substrates. Journal of Tropical Forestry and Environment, 7(1), pp. 65–73. Available at: https://doi.org/10.31357/jtfe.v7i1.3023.

Estrada, A.E.R. and Pecchia, J. (2017). Cultivation of Pleurotus ostreatus. Edible and Medicinal Mushrooms: Technology and Applications, pp. 339–360. Available at: https://doi.org/10.1002/9781119149446.ch16.

Ferial, Syaiful, S.A. and Dachlan, A. (2020). Growth of F0 Seedlings of Oysters Mushroom (Pleurotus ostreatus) with Different Ages of Explants. IOP Conference Series: Earth and Environmental Science, 486(1), pp. 1–7. Available at: https://doi.org/10.1088/1755-1315/486/1/012092.

Gupta, S., B. Summuna, M. Gupta, S.K. Annepu. (2019). Edible Mushrooms: Cultivation, Bioactive Molecules, and Health Benefits. Reference Series in Phytochemistry. Available at: https://doi.org/10.1007/978-3-319-78030-6_86.

Heredia-Olea, E., Contreras-Alvarado, M. D., Perez-Carrilo, E., Rosa-Millian, J. D., Serna-Saldivar, S. O (2020). Assessment of The Techno-Functionality, Starch Digestion Rates and Protein Quality of Rice Flour–Whey Protein Instant Powders Produced in A Twin Extruder. International Journal of Food Science and Technology, 55(2), pp. 878–890. Available at: https://doi.org/10.1111/ijfs.14376.

Houette, T., Maurer, C., Niewiarowsli, R., Gruber, P. (2022). Growth and Mechanical Characterization of Mycelium-Based Composites towards Future Bioremediation and Food Production in the Material Manufacturing Cycle. Biomimetics, 7(3). Available at: https://doi.org/10.3390/biomimetics7030103.

Kurnia, F., Periadnadi, dan Nurmiati. (2020). Isolation of Schizophyllum commune from Rubberwood. International Journal of Current Microbiology and Applied Sciences, 9(12), pp. 1202–1207. Available at: https://doi.org/10.20546/ijcmas.2020.912.147.

Lestari, A., dan Jajuli, M. 2019. Isolasi, karakterisasi, dan produksi inokulan jamur merang (Volvariella volvaceae bull. Ex. Fr) sing dari beberapa lokasi budidaya di Karawang. Jurnal Agrotek Indonesia 2 (1). Pp. 54 – 59

Manzoor, A. Pandey, V. K., Dar, A. H., Fayaz, U., Dash, K. K., Shams, R., Ahamd, S., Bashir, I., Fayaz, J., Singh, P., Khan, S. A., Ganaie, T. A. (2023). Rice Bran: Nutritional, Phytochemical, and Pharmacological Profile and Its Contribution to Human Health Promotion. Food Chemistry Advances, 2, p. 1–12 . Available at: https://doi.org/10.1016/j.focha.2023.100296.

Niego, A., S. Rapior, N. Thongklang, W. Jaidee, S. Lumyong, K. Hyde. (2021). Macrofungi as a Nutraceutical Source: Promising Bioactive Compounds and Market Value. Journal of Fungi, 7(5). Available at: https://doi.org/10.3390/jof7050397.

Nurlita, A. I., Putra, I. P., Ikhsan, M. (2021). Catatan Pemanfaatan Schizophyllum commune di Kampung Udapi Hilir, Papua Barat. Integrated Lab Journal, 18(28), pp. 18–28.

Prasad, D., Singh, R. P., Gangwar, S. K. 2022. Mushroom culture: media, preparation and maintenance. Society for Science and Nature. Pp.17-22

Rahayu, S. and Martono, D.S. (2019). Optimizing Quality of White Oyster Mushroom Seeds Through Plant Propagation. IOP Conference Series: Earth and Environmental Science, 347(1). Available at: https://doi.org/10.1088/1755-1315/347/1/012027.

Roja, M. Thiribhuvanamala, G., Angappan K., Geetha, P., Amirtham, D. (2022). Cultural and Nutritional Requirements for the Growth of Medicinal Mushroom Schizophyllum commune Fr. Biological Forum-An International Journal. 14(2), pp. 32–37. Available at: http://www.researchtrend.net/.

Rosnan, N.D., Chuen, N.L. and Ngadin, A.A. (2019). First Record of In Vitro Growth Evaluation of Wild Mushroom, Schizophyllum commune from Pulau Kapas in Malaysia,” Asian Journal of Agriculture and Biology, 7(4), pp. 602–609.

Saetang, N., Amornlerdpison D., Rattanapot, T., Rumaraj R., Unpaprom, Y., (2022). Processing of Split Gill Mushroom as a Biogenic Material for Functional Food Purpose. Biocatalysis and Agricultural Biotechnology, 41. Available at: https://doi.org/10.1016/j.bcab.2022.102314.

Schoder, K.A., Krumpel, J., Muller, J., Lemmer, A (2024). Effects of Environmental and Nutritional Conditions on Mycelium Growth of Three Basidiomycota. Mycobiology, 52(2), pp. 124–134. Available at: https://doi.org/10.1080/12298093.2024.2341492.

Setiati, Y., Chaidir, L., Saputri, A., Roosda, A.A. (2019). Utilization of Rice Wastewater as an alternative Medium for Growth and Quality of Shiitake Mushrooms (Lentinula edodes). Journal of Physics: Conference Series, 1402(3), pp. 1–6. Available at: https://doi.org/10.1088/1742-6596/1402/3/033034.

Siwulski, M., Rzymski, A., Budka, P., Kalac, S., Budzynska, L., Dadowicz, E., Hajduk, L., Kozak, J., Budzulak, K., Sobieralski, P., Niedzielski (2019). The effect of Different Substrates on The Growth of Six Cultivated Mushroom Species and Composition of Macro and Trace Elements In Their Fruiting Bodies. European Food Research and Technology, 245(2), pp. 419–431. Available at: https://doi.org/10.1007/s00217-018-3174-5.

Sornlake, W., Rattanaphanjak, P., Champreda, V., Eurwilaichitr L., Kittisenachai S., Roytrakul S., Fujii T., Inoue H. (2017). Characterization of Cellulolytic Enzyme System of Schizophyllum commune Mutant And Evaluation of Its Efficiency on Biomass Hydrolysis. Bioscience, Biotechnology and Biochemistry, 81(7), pp. 1289–1299. Available at: https://doi.org/10.1080/09168451.2017.1320937.

Steinberg, G., Penalva, M. A., Riquelme, M., Wosten, H. A (2017). Cell Biology of Hyphal Growth. The Fungal Kingdom, 5(2), pp. 231–265. Available at: https://doi.org/10.1128/9781555819583.ch11.

Suwandecha, T. and Pisuchpen, S. (2024) . Characterization and Performance Evaluation of Mycelium-Based Biofoams for Cushioning Materials Using Edible Mushrooms. Journal of Renewable Materials, 12(11), pp. 1811–1836. Available at: https://doi.org/10.32604/jrm.2024.056334.

Yang, L., Hu, X. and Qin, Z. (2024). Stiff Substrate Increases Mycelium Growth Rate On Surface. MRS Bulletin, 49(12), pp. 1205–1216. Available at: https://doi.org/10.1557/s43577-024-00762-1.

Zhou, S. et al. (2016). Carbohydrate Changes During Growth and Fruiting in Pleurotus ostreatus. Fungal Biology, 120(6–7), pp. 852–861. Available at: https://doi.org/10.1016/j.funbio.2016.03.007.