Effect Of Solvent Polarity On The Phytochemical Profile And Bioactive Metabolite Of Sargassum

Muliawati Handayani; Nuning Mahmudah Noor; Pindo Witoko; Upy Raudatul Jannah

doi:10.25181/jppt.v26i2.4786

Authors

Muliawati Handayani Politeknik Negeri Lampung
Nuning Mahmudah Noor Politeknik Negeri Lampung
Pindo Witoko Politeknik Negeri Lampung
Upy Raudatul Jannah Politeknik Negeri Lampung

DOI:

https://doi.org/10.25181/jppt.v26i2.4786

Abstract

Sargassum is recognized as a valuable source of bioactive secondary metabolites; however, quantitative comparisons of solvent-dependent extraction efficiencies remain limited. The present study rigorously assesses the extraction performance of methanol and hexane for phenolics, tannins, and major phytochemical constituents. Samples were subjected to seven-day maceration followed by phytochemical screening, determination of total phenolics using the Folin–Ciocalteu method, and quantification of total tannins with a tannic acid standard. Methanol exhibited a markedly higher extraction yield (4.23%) compared with hexane (1.13%). Furthermore, methanol extracts contained substantially greater levels of phenolics (16.13 mg GAE/g) and tannins (36.86 mg TAE/g) than those obtained with hexane, which yielded only 0.26 mg GAE/g and 0.42 mg TAE/g, respectively. The resulting phytochemical profiles highlight a clear polarity-driven selectivity, wherein methanol preferentially extracts polar metabolites, while hexane favors non-polar constituents. Collectively, these findings demonstrate that methanol is the superior solvent for obtaining Sargassum extracts enriched with bioactive compounds.

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References

Alamsyah, H. K., Widowati, I. and Sabdono, A. (2014) ‘Aktivitas Antibakteri Ekstrak Rumput Laut Sargassum cinereum (J.G. Agardh) dari Pulau Panjang Perairan Jepara Terhadap Bakteri Escherichia coli dan Staphylococcus epidermidis’, Journal of Marine Research., 3(2), pp. 69–78.

Aryatikta, R. A., Winarni, S. and Pramono, S. N. (2022) ‘Kajian Pustaka Potensi Sargassum sp. sebagai Nutrasetikal’, Food Scientia Journal of Food Science and Technology, 2(2), pp. 139–159. doi: DOI: 10.33830/fsj.v2i1.3083.2022.

Atkins, P. and Jones, L. ta (2004) ‘Chemical Principles: The Quest for Insight fourth Edition’, in Chemical Bond.

Chew, Y. L. et al. (2008) ‘Antioxidant Activity of Three Edible Seaweeds from Two Areas in South East Asia’, ScienceDirect, 41, pp. 1067–1072. doi: 10.1016/j.lwt.2007.06.013.

Dewi, M. C., Kusumaningtyas, N. M. and Kurniawan (2021) ‘Studi Pengaruh Variasi Konsentrasi Pelarut Maserasi Terhadap Kadar Senyawa Flavonoid Teh Hijau (Camelia Sinensis)’, Jurnal of Islamic Pharmacy, 5(Maret), pp. 67–72.

Edison et al. (2020) ‘Komponen Bioaktif dan Aktivitas Antioksidan Ekstrak Kasar Sargassum plagyophyllum’, JPHPI, 23, pp. 58–66.

Ellyya, S., Rosmawaty, P. and Endang, S. (2015) ‘Purification and characteriszation of Fucoidan from the Brown Seaweed Saegassum Binder Sorder’, Aqualen Bull oF Mar & Fiah. Postharvest & Biotech, 10, pp. 79–87.

FAO (2000) Quantification of Tannins in Tree Foliage Quantification of Tannins in Tree Foliage. Vienna: Joint FAO/ IAEA Division Of Nuclear Techniques Infood and Agriculture.

Ghaisani, A. D., Setyaningsih, I. and Hardiningtyas, S. D. (2025) ‘The Effect of Extraction Methods on Phenolic Content, Antioxidant Activity and Compound Identification of Spirulina platensis’, JPHPI 2025, 28(7), pp. 618–632. doi: http://dx.doi.org/10.17844/jphpi. v28i7.64276.

Jannah, M., Hanapi, A. and Fasya, G. (2014) ‘Uji Toksisitas dan Fitokimia Ekstrak Kasar Metanol, Kloroform dan N- Heksana Alga Coklat Sargassum vulgare Dari Pantai Kapong Pamekasan Madura’, Alchemy, 3(Oktober), pp. 194–203.

Kabir, M. M. et al. (2025) ‘Exploring the Potential of Lipid Compounds from Seaweeds in Enhancing Sun Protecting Factor ( SPF) : A Literature Review’, Jurnal Kesehatan Tambusai, 6(Juni), pp. 7058–7071.

Malangngi, L. P., Sangi, M. S. and Paendong, J. J. E. (2012) ‘Penentuan Kandungan Tanin dan Uji Aktivitas Antioksidan Ekstrak Biji Buah Alpukat (Persea americana Mill.)’, Jurnal MIPA Unstrat, 1(1), pp. 5–10.

Maureen, D. et al. (2017) ‘Seasonal Variation of Sargassum Muticum Biochemical Composition Determined by Fourier Transform Infra-Red Spectroscopy’, Journal of Analytical, Bioanalytical and Separation Techniques, 2(September), pp. 1–10. doi: 10.15436/2476-1869.17.1555.

Nurhayati, Siadi, K. and Harjono (2012) ‘Pengaruh Konsentrasi Natrium Benzoat dan Lama Penyimpanan pada Kadar Fenolat Total Pasta Tomat’, Indonesian Journal of Chemical Science, 1(2), pp. 158–163.

Putri, M. P. and Setiawati, Y. H. (2015) ‘Analisis Kadar Vitamin C Pada Buah Nanas Segar (Ananas comosus (L.) Merr) dan Buah Nanas Kaleng dengan Metode Spektrofotometri UV-VIS’, Jurnal Wiyata, 2(Juni), pp. 34–38.

Rebaya, A. et al. (2015) ‘Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae)’, Journal of Applied Pharmaceutical Science, 5(01), pp. 52–57. doi: 10.7324/JAPS.2015.50110.

Riskiana, N. P. Y. C. and Vifta, R. L. (2021) ‘Kajian Pengaruh Pelarut Terhadap Aktivitas Antioksidan Alga Coklat Genus Sargassum dengan Metode DPPH’, Journal of Holistics and Health Sciences, 3(2), pp. 201–213.

Rorong, J. A. (2008) ‘Uji Aktivitas Antioksidan dari Daun Cengkeh (Eugenia Carryophyllus) dengan Metode DPPH’, Chem. Prog., 1(2), pp. 111–116.

Sari, A. P., Yudiati, E. and Sunaryo (2020) ‘Toksisitas Partisi N-Heksan dan Etil Asetat pada Ekstrak Sargassum sp. Terhadap Larva Aedes aegypti Instar III’, Journal of Marine Research, 9(2), pp. 143–150.

Sulistiawanti, N. L. et al. (2025) ‘Review : Perbedaan Penggunaan Pelarut Terhadap Nilai Rendemen yang Dihasilkan dengan Berbagai Metode Ekstraksi dalam Proses Ekstraksi’, Jurnal Ilmu Farmasi dan Kesehatan, 3(Agustus), pp. 213–231. doi: https://doi.org/10.59841/an-najat.v3i3.3169.

Sultana, B., Anwar, F. and Ashraf, M. (2009) ‘Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts’, Molecules, 14(Juni), pp. 2167–2180. doi: 10.3390/molecules14062167.

Swetha, K., Uma, M. and Illanjiam (2024) ‘Phyto-chemical Profiling , Antimicrobial and Antioxidant Activity of Marine Brown Algae, Sargassum polycystum’, International Journal of Ecology and Environmental Sciences, 50(4), pp. 623–629. doi: https://doi.org/10.55863/ijees.2024.0205.

Tierney, M. S. et al. (2013) ‘Influence of Pressurised Liquid Extraction and Solid – Liquid Extraction Methods on The Phenolic Content and Antioxidant Activities of Irish Macroalgae’, International Journal of Food Science and Technology, 48(September), pp. 860–869. doi: 10.1111/ijfs.12038.

Tiwari, P. et al. (2011) ‘Phytochemical Screening and Extraction: A Review’, Internationale Pharmaceutica Sciencia, 1(1), pp. 98–106.

Yende, S. R., Harle, U. N. and Chaugule, B. B. (2014) ‘Therapeutic Potential and Health Benefits of Sargassum Species’, Pharmacognosy Reviews, 8(15). doi: 10.4103/0973-7847.125514.

Yuni, E., Ishak and Sylvia, N. (2021) ‘Ekstraksi Minyak Serai Dapur (Cymbopogon Citratus) Menggunakan Metode Maserasi’, Jurnal Teknologi Kimia Unimal, 10(November), pp. 57–70.

Zou, Y., Lu, Y. and Dongzhi, W. (2004) ‘Antioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L . in Vitro’, Journal of Agricultural and Food Chemistry, 52, pp. 5032–5039.