1,2,3-propanetriol: A Secondary Metabolite Compound of Rice (Oryza sativa L.) Rhizobacteria with Potential as a Bioelicitor
DOI:
https://doi.org/10.25181/jppt.v26i3.4341Keywords:
Glycerol, Microorganisms, Phytostimulator, Plant Resistance. Rhizosphere.Abstract
Rhizosphere microorganisms (Plant Growth Promoting Rhizobacteria) play an important role in the plant nutrient cycle (fixation, solubilization, and nutrient supply), phytostimulator production, and plant resistance to pathogens. This study aims to identify the types of rice rhizosphere PGPR and examine secondary metabolite compounds from microorganisms that have the potential as elicitors. This study was conducted using observation and descriptive analysis methods by comparing inorganic and organic rice rhizosphere PGPR. Inorganic-rice rhizosphere soil samples were taken at Kebonsari, while organic ones were taken at Sumberjambe. The results showed that there were three types of bacteria, namely Azotobacter sp., Acetobacter sp., and Bacillus subtilis, for the inorganic rice rhizosphere, while in the organic rhizosphere there were also three types of bacteria, namely Bacillus aerophilus, Acetobacter sp., and Azotobacter sp. The GC-MS test results showed that the highest chemical compounds in the inorganic rice rhizosphere were acetic acid (CAS) and 2-pyrrolidinone, while the highest chemical compounds in the organic rice rhizosphere they were acetic acid (CAS) and 1,2,3-propanetriol. The 1,2,3-propanetriol compound (glycerol) has the potential to be an elicitor, while CAS is an antimicrobial compound.
Downloads
References
Adigun, O. A., Nadeem, M., Pham, T. H., Jewell, L. E., Cheema, M., & Thomas, R. 2021. Recent advances in bio‐chemical, molecular and physiological aspects of membrane lipid derivatives in plant pathology. Plant, cell & environment, 44(1), 1-16. https://doi.org/10.1111/pce.13904.
Basma, N. S., Headen, T. F., Shaffer, M. S., Skipper, N. T., & Howard, C. A. 2018. Local structure and polar order in liquid N-methyl-2-pyrrolidone (NMP). The Journal of Physical Chemistry B, 122(38), pp 8963-8971. DOI: 10.1021/acs.jpcb.8b08020.
Christianson, D. W. 2017. Structural and chemical biology of terpenoid cyclases. Chemical reviews, 117(17), pp 11570-11648. https://doi.org/10.1021/acs.chemrev.7b00287.
Diarti, M. W., Yuri, S., & Yunan, J. 2017. Karakteristi.k morfologi, koloni dan biokimia bakteri yang diisolasi dari sedimen laguna perindukan nyamuk. Jurnal Kesehatan Prima, 11(2), pp 124-136.
Elhany, N. A., & Husnudin, U. B. 2023 Isolasi dan karakterisasi bakteri tahan logam berat pada perairan sungai Driyorejo Gresik. Jurnal Biogenic, 1(1), pp 28-33. https://doi.org/10.36841/biogenic.v1i1.2899.
Ester, S. R., & Mukarlina, M. 2022. Bakteri asam asetat yang diisolasi dari cuka daging buah pisang mas (Musa acuminata L.). Biologica Samudra, 4(2), 76-87. pp 81-85. https://doi.org/10.33059/jbs.v4i2.4230.
Etesami, H., Jeong, B. R., & Glick, B. R. 2023. Potential use of Bacillus spp. as an effective biostimulant against abiotic stresses in crops—A review. Current Research in Biotechnology, 5, 100128. https://doi.org/10.1016/j.crbiot.2023.100128.
Fadia, A. A. 2022. Pengaruh Asap Cair Sekam Padi Terhadap Hama Ulat Grayak (Spodoptera Frugiperda) Pada Tanaman Jagung (Zea Mays) (Doctoral dissertation, Politeknik Negeri Jember).
Gingichashvili, S., Duanis-Assaf, D., Shemesh, M., Featherstone, J. D., Feuerstein, O., & Steinberg, D. 2019. The adaptive morphology of Bacillus subtilis biofilms: a defense mechanism against bacterial starvation. Microorganisms, 8(1), 62. https://doi.org/10.3390/microorganisms8010062.
Gurikar, C., Sreenivasa, M. Y., Gowda, N. N., & Lokesh, A. C. 2022. Azotobacter sp.—A potential symbiotic rhizosphere engineer. In Rhizosphere Engineering. Pp 97-112. Academic Press. https://doi.org/10.1016/B978-0-323-89973-4.00010-7
Hardiansyah, M. Y., Musa, Y., & Jaya, A. M. 2020. Identifikasi plant growth promoting rhizobacteria pada rhizosfer bambu duri dengan gram KOH 3%. Agrotechnology Research Journal, 4(1), pp 41-46. https://doi.org/10.20961/agrotechresj.v4i1.40875.
Hermiati, H., Nurtjahya, E., & Mansur, I. 2021. Abundance and potency of Non-Symbiotic Nitrogen-Fixing Bacteria in Padang Sapu-sapu, Pejem Village, Bangka. Berkala Sainstek, 9(2), pp 95-102. https://doi.org/10.19184/bst.v9i2.20057.
Hindersah, R., Suryatmana, P., Fitriatin, B. N., Asmiran, P.., Nurbaity, A., & Setiawati, M. R. 2024. Bacterial-coated urea to reduce the use of urea fertilizer in potato cultivation. Jurnal Penelitian Pertanian Terapan, 24(1), pp 78–84. https://doi.org/10.25181/jppt.v24i1.2750.
Hutomo, P. A. B. 2023. Karakter agronomi dan fisiologi tanaman padi (Oryza sativa L. Cv inpari unsoed 79 agritan) yang terinfeksi Xanthomonas oryzae pv. Oryzae dan diinokulasi rhizobacteria indigenous lahan salin. Jurnal Penelitian Pertanian Terapan, 23(1) pp 13-24. DOI:10.25181/jppt.v23i1.2215.
Iqbal, S., Begum, F., Rabaan, A. A., Aljeldah, M., Al Shammari, B. R., Alawfi, A., Alshengeti, A., Sulaiman, T., & Khan, A. 2023. Classification and multifaceted potential of secondary metabolites produced by Bacillus subtilis. Group: A Comprehensive Review. Molecules (Basel, Switzerland), 28(3), 927. https://doi.org/10.3390/molecules28030927.
Jufri, S. W., Restu, M., & Gusmiaty, G. 2017. Identifikasi dan karakterisasi mikroorganisme rhizosfer pada hutan rakyat tanaman bitti (Vitexcofassus reinw), jati (Tectona grandis) dan jabon merah (Anthocephalus macrophyllus). Universitas Hasanuddin.
Kalam, S., Das, S. N., Basu, A., & Podile, A. R. 2017. “Population densities of indigenous Acidobacteria change in the presence of Plant Growth Promoting Rhizobacteria (PGPR) in rhizosphere”. Journal of Basic Microbiology. 57(5): 376–385. https://doi.org/10.1002/jobm.201600588.
Kim, S. T., Schatz, A., & Choi, C. 2021. Safety and toxicology of pyrrolidones and pyrrolidone‐based polymers. Handbook of pyrrolidone and caprolactam based materials: Synthesis, Characterization and Industrial Applications, 6 Volume Set, 2137-2202. pp 2-3. https://doi.org/10.1002/9781119468769.hpcbm027.
Kunti Setiowati, F. 2021. Produksi dan profil komponen senyawa bioaktif organosulfur pada kultur suspensi sel bawang putih (Allium sativum L.) Tunggal sebagai respon terhadap pemberian prekursor dan elisitor. (Doctoral dissertation, Universitas Brawijaya).
Maatoke, C. D., Dewani, Z., & Suciati, F. 2024. Karakterisasi morfologi dan fisiologi mikrob pelarut fosfat dan mikrob penambat n2 (Azotobacter sp.) dari rhizosfer tanaman padi dan tanah hutan cifor dramaga bogor. Bio-Lectura: Jurnal Pendidikan Biologi, 11(1), pp 115-117. https://doi.org/10.31849/bl.v11i1.19518.
Marom, N., Rizal, F., & Bintoro, M. 2017. Uji efektivitas saat pemberian dan konsentrasi PGPR (Plant Growth Promoting Rhizobacteria) terhadap produksi dan mutu benih kacang tanah (Arachis hypogaea L.). Agriprima : Journal of Applied Agricultural Sciences, 1(2), pp 174–184. DOI: 10.25047/agriprima.v1i2.43.
Mathesius U. 2018. Flavonoid functions in plants and their interactions with other organisms. Plants (Basel, Switzerland), 7(2), 30. https://doi.org/10.3390/plants7020030
Mohanty, P., Singh, P.K., Chakraborty, D. Mishra, S. Pattnaik, R. 2021. Insight into the role of PGPR in sustainable agriculture and environment. Frontiers in Sustainable Food Systems, 5, pp 1-12. https://doi.org/10.3389/fsufs.2021.667150.
Muis, A., N. Nonci, N. Djaenuddin. 2015. Evaluasi lima jenis inert carrier dan formulasi Bacilus subtilis untuk pengendalian hawar pelepah jagung (Rhizoctonia solani Kuhn) J. HPT Tropika. 15(2): pp 164–169. https://doi.org/10.23960/j.hptt.215164-169.
Muriady, Meilina, H. dan Faisal, M. 2022. Antibacterial activity of liquid smoke powder from rice husk, International Journal of GEOMATE, 23(95), pp. 89–96. DOI: https://doi.org/10.21660/2022.95.7522.
Muriady, Meilina, H. dan Faisal, M. 2022. Antibacterial Activity of Liquid Smoke Powder From Rice Husk, International Journal of GEOMATE, 23(95), pp. 89–96. DOI: https://doi.org/10.21660/2022.95.7522.
Mwendwa, P. K., Karanja, A. W., & Maingi, J. M. 2023. Endophytic Bacillus aerophilus from the Leaves of Azadirachta indica as a Potential Biocontrol against Staphylococcus aureus. Journal of Advances in Microbiology, 23(10), pp 116–127. https://doi.org/10.9734/jamb/2023/v23i10762
Nuraini, C., Saida, S., Suryanti, S., & Nontji, M. 2020. Isolasi Dan Identifikasi Bakteri Rhizosfer Tanaman Jagung Pada Fase Vegetatif Dan Generatif. AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 1(1), pp 24-30. DOI: https://doi.org/10.33096/agrotekmas.v1i1.103.
Nurhaen, N., Winarsii, D., & Ridhay, A. 2016. Isolasi dan identifikasi komponen kimia minyak atsiri dari daun, batang dan bunga tumbuhan salembangu (Melissa sp.). Natural Science: Journal of Science and Technology, 5(2). DOI:10.22487/25411969.2016.v5.i2.6702.
Ogawa, D., Suzuki, Y., Yokoo, T. et al 2021. Acetic-acid-induced jasmonate signaling in root enhances drought avoidance in rice. Sci Rep 11, 6280. https://doi.org/10.1038/s41598-021-85355-7.
Ogawa, D., Suzuki, Y., Yokoo, T. et al 2021. Acetic-acid-induced jasmonate signaling in root enhances drought avoidance in rice. Sci Rep 11, 6280 (2021). https://doi.org/10.1038/s41598-021-85355-7
Panjaitan, D., Wardhana, V. W., Hadi, R., Tsuraya, F., & Naibaho, F. G. 2023. Pelatihan karakterisasi morfologi bakteri dan fungi sebagai pengayaan praktikum biologi bagi guru sekolah menengah atas. JMM (Jurnal Masyarakat Mandiri), 7(1), pp 556-565. https://doi.org/10.31764/jmm.v7i1.12355.
Prabawati, A., & Susilowati, A. Sugiyarto. 2019. Bakteri filosfer padi sebagai kandidat agen biokontrol terhadap Xanthomonas oryzae pv. oryzae (Xoo) penyebab penyakit hawar daun bakteri. In Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia Vol. 5, No. 2, pp. 256-262. https://doi.org/10.13057/psnmbi/m050219.
Pratama, T., & Sarmila, S. 2022. Potensi Bacillus subtilis dalam mengendalikan penyakit busuk bulir bakteri (Bulkholderia glumae) tanaman padi (Oryza Sativa L.). Tarjih Agriculture System Journal, 2(1), pp 95-100. Retrieved from https://mail.jurnal-umsi.ac.id/index.php/agriculture/article/view/373.
Primastoeti, T. 2020. Identifikasi Dan Analisis Potensi Isolat Bakteri Endofit Sebagai Mikrobia Pendukung Pertumbuhan Tanaman Dari Tanaman Padi (Oryza sativa). (Doctoral dissertation, Universitas Gadjah Mada).
Puspawati, N. M. I., Khalimi, K., & Wirya, G. N. A. S. 2021. Pemanfaatan Bakteri Azotobacter sp. untuk Meningkatkan Efisiensi Penggunaan Pupuk Urea pada Tanaman Padi (Oryza sativa L.). Jurnal Agroekoteknologi Tropika ISSN, 2301, 6515, pp 338-398.
Rosmania, R., & Yanti, F. 2020. Perhitungan jumlah bakteri di Laboratorium Mikrobiologi menggunakan pengembangan metode Spektrofotometri. Jurnal Penelitian Sains, 22(2), pp 76-86. ttps://doi.org/10.56064/jps.v22i2.564.
Santoso, R. 2021. Analisis Kandungan Vitamin C Dan Aktivitas Antioksidan Teh Kombucha Berdasarkan Lama Fermentasi Dan Jenis Teh. (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim).
Saxena, A. K., Kumar, M., Chakdar, H., Anuroopa, N., & Bagyaraj, D. J. 2020. Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology, 128(6), pp 1583-1594. https://doi.org/10.1111/jam.14506.
Semiun, C. G. 2022. Karakterisasi Bakteri Akar Padi Sawah (Oryza Sativa L.) Desa Noelbaki, Kabupaten Kupang. Indigenous Biologi: Jurnal Pendidikan dan Sains Biologi, 5(1), pp 15-24. https://doi.org/10.33323/indigenous.v5i1.293.
Stevenson, A., Hamill, P. G., Medina, Á., Kminek, G., Rummel, J. D., Dijksterhuis, J., ... & Hallsworth, J. E. (2017). Glycerol enhances fungal germination at the water‐activity limit for life. Environmental Microbiology, 19(3), pp 947-967. https://doi.org/10.1111/1462-2920.13530.
Sukmadewi, D. K. T., Suharjono, & Antonius, S. 2015. Uji Potensi Bakteri Penghasil Hormon IAA (Indole Acetic Acid) dari Tanah Rhizosfer Cengkeh (Syzigium aromaticum L.). Jurnal Biotropika, 3(2), pp 91–94. https://biotropika.ub.ac.id/index.php/biotropika/article/view/361.
Sumi, C. D., Yang, B. W., Yeo, I. C., & Hahm, Y. T. 2015. Antimicrobial peptides of the genus Bacillus a new era for antibiotics. Canadian Journal of Microbiology, 61(2), pp 93-103. https://doi.org/10.1139/cjm-2014-0613.
Vlot, A. C., Sales, J. H., Lenk, M., Bauer, K., Brambilla, A., Sommer, A., ... & Nayem, S. 2021. Systemic propagation of immunity in plants. New Phytologist, 229(3), 1234-1250. https://doi.org/10.1111/nph.16953.
Wang, W., Li, Y., Dang, P., Zhao, S., Lai, D., & Zhou, L. 2018. Rice Secondary Metabolites: Structures, Roles, Biosynthesis, And Metabolic Regulation. Molecules, 23(12), 3098. doi: 10.3390/molecules23123098
Wisdawati, E., Kuswinanti, T., Rosmana, A., & Nasruddin, A. 2019. Keanekaragaman cendawan rizosfer pada tanaman talas satoimo. Agroplantae: Jurnal Ilmiah Terapan Budidaya dan Pengelolaan Tanaman Pertanian dan Perkebunan, 8(2), 51-57. Education, Sains and Technology), 6(2), pp 51-57.
Xiao, H., Zhang, Y., & Wang, M. 2019. Discovery and engineering of cytochrome P450s for terpenoid biosynthesis. Trends in Biotechnology, 37(6), 618-631.
Yuan, A., Kumar, S. D., Wang, H., Wang, S., Impa, S., Wang, H., ... & Shao, R. 2024. Dynamic interplay among soil nutrients, rhizosphere metabolites, and microbes shape drought and heat stress responses in summer maize. Soil Biology and Biochemistry, 191, 109357. https://doi.org/10.1016/j.soilbio.2024.109357.
Yulistiana, E., Widowati, H., & Sutanto, A. 2020. Plant growth promoting rhizobacteria (PGPR) dari akar bambu apus (Gigantochloa apus) meningkatkan pertumbuhan tanaman. Biolova, 1(1), pp 1–6. https://doi.org/10.24127/biolova.v1i1.23
Zhang, Y., Smith, P., Maximova, S. N., & Guiltinan, M. J. 2015. Application of glycerol as a foliar spray activates the defence response and enhances disease resistance of T heobroma cacao. Molecular Plant Pathology, 16(1), pp 27-37. https://doi.org/10.1111/mpp.12158.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Jurnal Penelitian Pertanian Terapan

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online
(e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
