Kandungan Total Fenol dan Aktivitas Antibakteri Kelopak Buah Rosela Merah dan Ungu Sebagai Kandidat Feed Additive Alami Pada Broiler

Abstract

Broiler farming always use feed additives commercial to improve productivity of
broiler, but feed additives commercial have negative effect for consument. Feed
additive should have ability as an antibacterial. To minimize using commercial
feed additive we have to find alternatives to optimize the use of roselle calyces.
This experiment was conducted to knowing phenol content and antibacterial
activity of red roselle (Hibiscus sabdariffa) calyces and purple roselle (Hibiscus
sabdariffa) calyces and to determine the type of roselle calyces which have
potential as feed additive candidate on broiler. The phenol content was conducted
using visible spectrophotometry method with Folin Ciocalteau reagen, and the
antibacterial activity using agar well method for measuring clearing zone againt
Staphylocccus aureus and Escherichia coli. The results showed that difference in
solvent temperature (300
C, 600
C and 900
C) of red and purple roselle calyces
didn’t affect the phenol content, but the extract 10% of purple roselle calyces
containing phenol content higher than the extract of red roselle calyces. The
difference of solvent temperature didn’t affect the antibacterial activity, but
extracts 10% of purple roselle calyces have antibacterial activity higher than red
roselle calyces. Purple roselle calyces more potencial as feed additive.

Keywords : Roselle (Hibiscus sabdariffa) calyces, phenol content, antibacterial
activity