Potensi Penggunaan UV-Visible Spectroscopy dan PLS-DA untuk Membedakan Kopi Luwak dan Bukan Luwak
DOI:
https://doi.org/10.25181/prosemnas.v0i0.484Abstrak
The process of discrimination between origin coffee and civet coffee is very important to prevent counterfeiting civet coffee, especially in the form of powder. In this study we used the method of UV-Visible Spectroscopy and PLS-DA method for classifying two types of coffee that civet coffee and regular coffee (not the mongoose) of Robusta coffee types. Spectral data retrieval is done using a UV-VIS / NIR spectrometer (Jasco Corp., Tokyo, Japan). PLS-DA method was then used to build the model calibration and validation for the determination of the type of coffee. The results show that the model calibration PLS-DA has a value of R2 = 0.99, indicating a very strong relationship between the actual and predicted the type of coffee. RMSEC also very small value of 0.05. Validation of calibration models using full cross validation method also produces the coefficient of determination (R2), which was very high at 0.99 with a bias that is very small in the amount -0004. These results indicate that the model of PLS-DA has been successfully built and tested to make the process of discrimination civet coffee and coffee instead mongoose with very satisfactory results.Keywords: Civet coffee, UV-Visible spectroscopy, discrimination,Unduhan
Referensi
Fredholm, B.B. 2011. Notes on the history of caffeine use. Dalam: Fredholm, B.B. (ed.). Methylxanthines,
Handbook of Experimental Pharmacology, hal 1-9. Springer-Verlag Berlin Heidelberg.
International Coffee Organization 2016. Total production by all exporting countries.
http://www.ico.org/prices/po-production.pdf. [22 Agustus 2016].
Souto, U.T.C.P., Barbosa, M. F., Dantas, H.V., Pontes, A.S., Lyra, W.S., Diniz, P.H.G.D., Araújo, M.C.U.,
dan Silva, E.C. 2015. Identification of adulteration in ground roasted coffees using UV–Vis
spectroscopy and SPA-LDA. LWT - Food Science and Technology 63(2): 1037–1041.
Suhandy, D., Yulia, M., Waluyo, S., Sugianti, C., Iriani, R., Handayani, F.N., dan Apratiwi, N. 2016a. The
feasibility of using ultraviolet-visible spectroscopy and soft independent modelling of class analogies
(SIMCA) for classification of Indonesian palm civet coffee (kopi luwak). Proceeding of The USR
international seminar on food security (UISFS). 23-25 Agustus 2016. Bandar Lampung.
Suhandy, D., Waluyo, S., Sugianti, C., Yulia, M., Iriani, R., Handayani, F.N., dan Apratiwi, N. 2016b. The
use of UV-Vis-NIR spectroscopy and chemometrics for identification of adulteration in ground
roasted arabica coffees -investigation on the influence of particle size on spectral analysis-. Prosiding
Seminar Nasional Tempe. 28 Mei 2016. Bandar Lampung.
Yulia, M., Suhandy, D., Waluyo, S., dan Sugianti, C. 2016. Detection and quantification of adulteration in
luwak coffee through ultraviolet-visible spectroscopy combined with chemometrics. Proceeding of
The USR international seminar on food security (UISFS). 23-25 Agustus 2016. Bandar Lampung.
