Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium

Authors

  • Dwi Erwin Kusbianto Universitas Jember
  • Sri Emiyati Universitas Jember
  • Tri Candra Setiawati Universitas Jember
  • Gatot Subroto Universitas Jember
  • Mohammad Ghufron Rosyady Universitas Jember

DOI:

https://doi.org/10.25181/jaip.v10i2.2550

Keywords:

auxin, microbial IAA, vanilla seedlings

Abstract

Propagation of vanilla plants is generally using vegetative methods like cuttings. The use of external growth regulators can support the growth of nurseries. Phosphate solubilising bacteria and potassium solubilising bacteria as plant growth promoting rhizobacteria are producing microbial IAA. This study used auxin in synthetic IAA combined with IAA microbes from phosphate solubilising bacteria and potassium solubilising bacteria. This study aimed to determine the effectiveness of each microbe at each dose of synthetic IAA that was applied. The study was conducted from November 2021-April 2022 in the Biological Soil Laboratory and Greenhouse University of Jember. The research uses a randomised block design with three replications with the factorial arrangement. The first factor was organic matter which was enriched with bacterial isolate (B0 = control, B1 = BPF isolate, and B2 = BPK isolate). The second factor was concentration of IAA (A0 = 0 mg.l-1, A1 = 100 mg.l-1, and A2 = 200 mg.l-1). The data were analysed by ANOVA and further tested with Duncan Multiple Range Test (DMRT) with a 95% confidence level. The results showed that treatment of synthetic IAA affects the parameters of length and volume of adventitious roots, which are effective in helping to supply nutrients with the best treatment at a concentration of 200 ppm. Meanwhile, enrichment of media with isolates of phosphate solubilising bacteria and potassium solubilising bacteria gave a better effect than control on the observation of tendril length, number of leaves, number of adventitious roots and taproots, length of adventitious roots and taproots, the volume of adventitious roots and taproot, and fresh weight of vanilla seedlings.

Downloads

Download data is not yet available.

References

Adugna, M., Belew, D., & Tilahun, D. (2015). Influence of rooting media and number of nodes per stem cutting on nursery ... mesay adugna. Journal of Horticulture and Forestry, 7(3), 48–56. https://doi.org/10.5897/JHF2014.0376

Ali, M. R., Rusmarini, U. K., & Setyowati, E. R. (2017). Pengaruh macam zat pemacu pertumbuhan dan pupuk organik terhadap pertumbuhan awal bibit kelapa sawit di pre nursery. Jurnal Agromat. 2(1), 1-16.

Babu, B. H., Larkin, A., & Kumar, H. (2018). Effect of plant growth regulators on rooting behavior of stem cuttings of Terminalia arjuna (ROXB.). Plant Archives, 18(2), 2159-2164.

Badan Pusat Statistik. (2012). Data luas areal tanaman perkebunan rakyat menurut jenis tanaman. diakses dari https://www.bps.go.id/indicator/54/770/3/luas-areal-tanaman-perkebunan-rakyat-menurut-jenis-tanaman.html. di akses pada tanggal 20 Desember 2021 pada pukul 21.25 WIB.

Badan Pusat Statistik. (2014). Data produksi perkebunan rakyat menurut jenis tanaman. diakses dari https://www.bps.go.id/indicator/54/768/3/produksi-perkebunan-rakyat-menurut-jenis-tanaman.html. di akses pada tanggal 20 Desember 2021 pada pukul 21.17 WIB.

Basu, A., Prasad, P., Das, S. N., Kalam, S., Sayyed, R. Z., Reddy, M. S., & Enshasy, H. El. (2021). Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: recent developments, constraints, and prospects. Sustainability, 13(3), 1140. https://doi.org/10.3390/SU13031140

Biswas, S., Shivaprakash, M. K., & Maina, C. C. (2018). Biosynthesis of phytohormones by potassium solubilising bacteria isolated from banana rhizosphere. International Journal of Agriculture, Environment and Biotechnology, 11(3), 497-501.

Druege, U., Hilo, A., Pérez-Pérez, J. M., Klopotek, Y., Acosta, M., Shahinnia, F., ... & Hajirezaei, M. R. (2019). Molecular and physiological control of adventitious rooting in cuttings: phytohormone action meets resource allocation. Annals of Botany, 123(6), 929-949.

Fantri, M. (2021). Pengaruh panjang rizoma dan aplikasi ZPT nabati terhadap pertumbuhan tanaman rami (Boehmeria nivea L. Gaud). Retrieved from https://sipora.polije.ac.id/id/eprint/9286

Gupitasari. T. P., Noli, Z. A., & Suwirman. (2019). Induksi akar dan pertumbuhan setek pucuk jirak (Eurya acuminate DC.) dengan pemberian zat pengatur tumbuh IBA, NAA, IAA pada berbagai media tanam. Metaformosa: Journal of Biological Sciences. 6(2), 268-275.

Hadipoentyanti, E., & Udarno, L. (1982). Botani Panili dalam Monograf Panili. Balai Penelitian Tanaman Rempah dan Obat.

Handayani, E., Palupi, T., Fadjar, D., Tingkat, R., Pertumbuhan, K., Lada, S., & Rianto, F. (2020). Tingkat keberhasilan pertumbuhan setek lada dengan aplikasi naungan dan berbagai hormon tumbuh auksin. Agrovigor: Jurnal Agroekoteknologi, 13(2), 106–111. https://doi.org/10.21107/AGROVIGOR.V13I2.6709

Hoesen, D., Hazar, S., Priyono, & Sumarnie, H. (2000). Peranan zat pengatur tumbuh IBA, NAA dan IAA pada perbanyakan amarilis merah (Amaryllidaceae). Prosiding Seminar Hari Cinta Puspa dan Satwa Nasional. Lab. Treub Laitbang Botani Puslitbang Biologi. LIPI: Bogor.

Isnaini. (2006). Pertanian Organik. Kreasi Wacana.

Ivanchenko, M. G., Napsucialy‐Mendivil, S., & Dubrovsky, J. G. (2010). Auxin‐induced inhibition of lateral root initiation contributes to root system shaping in Arabidopsis thaliana. The Plant Journal, 64(5), 740-752.

Jamaludin, J., & Ranchiano, M. G. (2021). Pertumbuhan tanaman vanili (Vanilla planifolia) dalam polybag pada beberapa kombinasi media tanam dan frekuensi penyiraman menggunakan teknologi irigasi tetes. Jurnal Agro Industri Perkebunan, 9(2), 65-72. https://doi.org/10.25181/jaip.v9i2.1867

Kadmiri, I. M., Chaouqui, L., Azaroual, S. E., Sijilmassi, B., Yaakoubi, K., & Wahby, I. (2018). Phosphate-solubilizing and auxin-producing rhizobacteria promote plant growth under saline conditions. Arabian Journal for Science and Engineering, 43(7), 3403-3415.

Lakehal, A., Chaabouni, S., Cavel, E., Le Hir, R., Ranjan, A., Raneshan, Z., ... & Bellini, C. (2019). A molecular framework for the control of adventitious rooting by TIR1/AFB2-Aux/IAA-dependent auxin signaling in Arabidopsis. Molecular Plant, 12(11), 1499-1514.

Laviendi, A., Ginting, J., & Irsal. (2017). Pengaruh perbandingan media tanam kompos kulit biji kopi dan pemberian pupuk NPK (15:15:15) terhadap pertumbuhan bibit kopi (Coffea arabica L.) di rumah kaca. Jurnal Agroteknologi FP USU, 5(1), 72-77.

Nakamura, M., Nishimura, T., & Morita, M. T. (2019). Bridging the gap between amyloplasts and directional auxin transport in plant gravitropism. Current Opinion Plant Biology, 52, 54–60. https://doi.org/10.1016/J.PBI.2019.07.005

Ramadhan, M., Setyorini, E., Rachmawati, N., & Andriati, E. (2019). Ayo Berkebun Vanili. Jakarta: Kementerian Pertanian. Retrieved from http://repository.pertanian.go.id/handle/123456789/9280

Rao, N. S. (1994). Mikroorganisme Tanah dan Pertumbuhan Tanaman. Edisi Kedua. UI-Press

Riaz, U., Murtaza, G., Anum, W., Samreen, T., Sarfraz, M., & Nazir, M. Z. (2021). Plant growth-promoting rhizobacteria (PGPR) as biofertilizers and biopesticides. Microbiota and Biofertilizers, 181–196. https://doi.org/10.1007/978-3-030-48771-3_11

Ruhnayat. A. (2003). Bertanam Vanili Si Emas Hijau Nan Wangi. Agromedia Pustaka.

Sarmiento-López, L., Quiroz-Figueroa, F. R., Sepúlveda-Jiménez, G., & Rodríguez-Monroy, M. (2022). Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor. Journal of Bioscience and Bioengineering. Retrieved from https://www.sciencedirect.com/science/article/pii/S1389172322000858

Setiawati, T. C., & Mutmainnah, L. (2016). Solubilization of potassium containing mineral by microorganisms from sugarcane rhizosphere. Agriculture and Agricultural Science Procedia, 9, 108-117.

Shi, B., & Vernoux, T. (2022). Hormonal control of cell identity and growth in the shoot apical meristem. Current Opinion in Plant Biology, 65, 102111.

Sudarmini, D. P., Sudana. I. M., Sudiarta, I. P., & Suastika, G. (2018). Pemanfaatan bakteri pelarut fosfat penginduksi hormone IAA (indol acetic acid) untuk peningkatan pertumbuhan kedelai (Glycine max). Jurnal Agric. Sci. and Biotechnol. 7(1), 1-12

Syavitri, D. A., Prayogo, C., & Gunawan, S. (2019). Pengaruh pupuk hayati terhadap pertumbuhan tanaman, dan populasi bakteri pelarut kalium pada tanaman tebu (Saccharum officinarum L.). Jurnal Tanah dan Sumberdaya Lahan, 6(2), 1341-1352.

Tien, L. H., Chac, L. D., Oanh, L. T. L., Ly, P. T., Sau, H. T., Hung, N., ... & Thinh, B. B. (2020). Effect of auxins (IAA, IBA and NAA) on clonal propagation of Solanum procumbens stem cuttings. Plant Cell Biotechnology and Molecular Biology, 21(55-56), 113-120.

Udarno, L., & Hadipoentyanti, E. (2009). Panili budidaya dan kerabat liarnya. Pengembangan Tanaman Industrial, 15(1), 27-28

Udia, B. A. A. A., Rusmin, D., Fatmawaty, A. A., Hermita, N., & Syukur, C. (2021). Mutu fisik dan fisiologis benih setek berkara vanili pada berbagai jenis media. Jurnal Kultivasi, 20(2), 111-119. https://doi.org/10.24198/kultivasi.v20i2.32698

Published

2022-10-10

How to Cite

Kusbianto, D. E., Emiyati, S., Setiawati, T. C., Subroto, G., & Rosyady, M. G. (2022). Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium. Jurnal Agro Industri Perkebunan, 10(2), 105-118. https://doi.org/10.25181/jaip.v10i2.2550

Issue

Section

Article