Identifikasi Isolat Rhizobakteria Indigenos Kandidat Agen Biokontrol Ganoderma boninense Berdasarkan Sekuen Gen 16S rRNA

Authors

  • Yulmira Yanti Universitas Andalas
  • Nurbailis Nurbailis Universitas Andalas
  • Imam Rifai Universitas Andalas

DOI:

https://doi.org/10.25181/jaip.v9i1.1494

Keywords:

basal stem rot, indigenous rhizobacteria, oil palm nursery management, 16S rRNA

Abstract

Rhizobacteria is a group of bacteria that colonize roots, affect growth and control plant pathogens. Based on the results of previous studies, 6 isolates have the best ability to control Ganoderma boninense in oil palm seedlings. It is important to characterize them molecularly. Molecular identifications of the selected rhizobacteria isolates were done using the 16S rRNA gene. The results showed that all isolates were identified to have similarity as 5 different species i.e Bacillus paramycoides, Microbacterium paraoxydans, B. albus, B. cereus, and Serratia marcescens based on NCBI database.

Downloads

Download data is not yet available.

Author Biographies

Yulmira Yanti, Universitas Andalas

proteksi Tanaman

Nurbailis Nurbailis, Universitas Andalas

Program Studi Proteksi Tanaman Fakultas Pertanian Universitas Andalas

Imam Rifai, Universitas Andalas

Pascasarjana Ilmu Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Andalas

References

Azizah, S. N., Mubarik, N. R., & Sudirman, L. I. (2015). Potential of chitinolytic Bacillus amyloliquefaciens SAHA 12 . 07 and Serratia marcescens KAHN 15 . 12 as biocontrol agents of Ganoderma boninense. Research Journal of Microbiology, 10(10), 452–465. https://doi.org/10.3923/jm.2015.452.465

Bakhtiar, Y., Yahya, S., & Sumaryono, W. (2012). Adaptation of oil palm seedlings inoculated with arbuscular mycorrhizal fungi and mycorrhizal endosymbiotic bacteria Bacillus subtilis B10 towards biotic stress of pathogen Ganoderma boninense Pat. Jurnal Microbiology Indonesia, 6(4), 157–164. https://doi.org/10.5454/mi.6.4.3

Beneduzi, A., Ambrosini, A., & Passaglia, L. M. P. (2012). Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology, 4, 1044–1051.

Bivi, M. R., Farhana, M. S., Khairulmazmi, A., & Idris, A. (2010). Control of Ganoderma boninense: A causal agent of basal stem rot disease in oil palm with endophyte bacteria in vitro. International Journal of Agriculture and Biology, 12(6), 833–839.

Bosshard, P. P., Abels, S., Zbinden, R., Böttger, E. C., & Altwegg, M. (2003). Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation). Journal of Clinical Microbiology, 41(9), 4134-4140.

Buana, R. F. N., Wahyudi, A. T., & Toruan-Mat, N. (2014). Control activity of potential antifungal-producing Burkholderia sp. in suppressing Ganoderma boninense growth in oil palm. Asian Journal of Agricultural Research, 8(5), 259–268. https://doi.org/10.3923/ajar.2014.259.268

Chen, Y. P., Rekha, P. D., Arun, A. B., Shen, F. T., Lai, W., & Young, C. C. (2006). Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology, 34, 33–41. https://doi.org/10.1016/j.apsoil.2005.12.002

Chong, K. P., Lum, M. S., Foong, C. P., Wong, C. M. V. L., Atong, M., & Rossall, S. (2011). First identification of Ganoderma boninense isolated from Sabah based on PCR and sequence homology. African Journal of Biotechnology, 10(66), 14718–14723. https://doi.org/10.5897/AJB11.1096

Cooper, R.M, Flood J., Rees R.W. 2011. Ganoderma boninense in oil palm plantations: Current thinking on epidemiology, resistance and pathology. Planter, 87(1024): 515–52.

Fredriksson, N. J., Hermansson, M., & Wilén, B. M. (2013). The choice of PCR primers has great impact on assessments of bacterial community diversity and dynamics in a wastewater treatment plant. PloS one, 8(10), e76431.

Glick B. R., Cheng, Z., Czarny, J., & Duan, J. (2007). Promotion of plant growth by ACC deaminase-producingsoil bacteria. Eur J Plant Pathol 119:329-39.

Idris, A., Kushairi, A., Ismail, S., & Ariffin, D. (2004). Selection for partial resistance in oil palm progenies to Ganoderma basal stem rot. J Oil Palm Res., 16(2), 12-18.

Kaur, G., Kaur, J., Dadhich, K. S., & Caemotra, S. S. (2011). Phosphate-solubilizing bacteria Microbacterium paraoxydans isolated from the rhizospheric system of wheat (Triticum aestivum). Transylv. Rev. Syst. Ecol. Res., 12, 149–161.

Khaeruni, A., Sutariati, G. A. K., & Rahman, A.. (2011). Potensi Rizobakteri indigenous Ultisol untuk mengendalikan penyakit busuk batang Phytophthora (Phytophthora capsici) pada tanaman cabai. Jurnal Agroteknos, 1(1), 9-15.

Li, F. X., Ma, H. Q., Liu, J., & Zhang, C. (2012). Antagonistic effects of Bacillus cereus strain B-02 on morphology, ultrastructure and cytophysiology of Botrytis cinerea. Polish Journal of Microbiology, 61(2), 119–128.

Ommelna, B. G., Jennifer, A. N, Chong, K. P. (2012). The potential of chitosan isuppressing Ganoderma boninense infection in oil palm seedlings. JSSM 7(2):186-19.

Petti, C. A., Polage, C. R., & Schreckenberger, P. (2005). The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. Journal of Clinical Microbiology, 43(12), 6123-6125.

Puspita, F, Zul, D., & Khoiri, A. (2013). Potensi Bacillus sp. asal rizosfer Giam Siak Kecil Bukit Batu sebagai rhizobacteria pemacu pertumbuhan dan antifungi pada pembibitan kelapa sawit. Seminar Nasional “Peranan Teknologi dan Kelembagaan Pertanian dalam Mewujudkan Pembangunan Pertanian yang Tangguh dan Berkelanjutan, 2009 (November), 7–15.

Roberts, D. P., McKenna, L. F., Lakshman, D. K., Meyer, S. L. F., Kong, H., de Souza, J. T., … Chung, S. (2007). Suppression of damping-off of cucumber caused by Pythium ultimum with live cells and extracts of Serratia marcescens N4-5. Soil Biology and Biochemistry, 39(9), 2275–2288. https://doi.org/10.1016/j.soilbio.2007.03.029

Rosselló-Mora R, & Amann R (2001) The species concept for prokaryotes. FEMS Microbiol Rev 25(1):39–67.

Sanderson, F. R. (2005). An insight into spore dispersal of Ganoderma boninense on oil palm. Mycopathologia, 159(1), 139–141. https://doi.org/10.1007/s11046-004-4436-2

Simmon, K. E., Mirrett, S., Reller, L. B., & Petti, C. A. (2008). Genotypic diversity of anaerobic isolates from bloodstream infections. Journal of Clinical Microbiology, 46(5), 1596-1601.

Suryanto, D., Wibowo, R. H., Siregar, E. B. M., & Munir, E.. (2012). A possibility of chitinolytic bacteria utilization to control basal stems disease caused by Ganoderma boninense in oil palm seedling. African Journal of Microbiology Research, 6(9). https://doi.org/10.5897/AJMR11.1343

Susanto, A., Prasetyo, A. E., Priwiratama, H., Wening, S., & Surianto, S. (2013). Ganoderma boninense penyebab penyakit busuk batang atas kelapa sawit. Jurnal Fitopatologi Indonesia, 9(4), 123-123. https://doi.org/10.14692/jfi.9.4.123

Tan, Z., Hurek, T., Vinuesa, P., Müller, P., Ladha, J. K., & Reinhold-Hurek, B. (2001). Specific detection of Bradyrhizobium and Rhizobium strains colonizing rice (Oryza sativa) roots by 16S-23S ribosomal DNA intergenic spacer-targeted PCR. Applied and Environmental Microbiology, 67(8), 3655-3664.

Vishwakarma, K., Kumar, V., Tripathi, D. K., & Sharma, S. (2018). Characterization of rhizobacterial isolates from Brassica juncea for multitrait plant growth promotion and their viability studies on carriers. Environmental Sustainability, 1(3), 253–265. https://doi.org/10.1007/s42398-018-0026-y.

Webster, G., Newberry, C. J., Fry, J. C., & Weightman, A. J. (2003). Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale. Journal of Microbiological Methods, 55(1), 155-164.

Yanti, Y., Arnetti, A., & Rifai, I. (2019). Penapisan isolat rizobakteri indigenos untuk pengendalian Ganoderma boninense pada bibit kelapa sawit (Elaeis guineensis Jacq.). Jurnal Agro Industri Perkebunan, 7(2), 59-68. https://doi.org/10.25181/jaip.v7i2.1156

Zahid, M., Abbasi, M. K., Hameed, S., & Rahim, N. (2015). Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.). Frontiers in Microbiology, 6, 1-10. https://doi.org/10.3389/fmicb.2015.00207

Published

2021-05-21

How to Cite

Yanti, Y., Nurbailis, N., & Rifai, I. (2021). Identifikasi Isolat Rhizobakteria Indigenos Kandidat Agen Biokontrol Ganoderma boninense Berdasarkan Sekuen Gen 16S rRNA. Jurnal Agro Industri Perkebunan, 9(1), 57-63. https://doi.org/10.25181/jaip.v9i1.1494

Issue

Section

Article