Uji Senyawa Taurin Sebagai Antikanker Terhadap Jumlah Sel-Sel Leukosit Dan Sel-Sel Eritrosit Mencit (Mus musculus L.) yang Diinduksi Benzo (Α) Pyren Secara In Vivo

Authors

  • Agra Maysa Jurusan Biologi – FMIPA Universitas Lampung – Bandar Lampung
  • E. L. Widiastuti Jurusan Biologi – FMIPA Universitas Lampung – Bandar Lampung
  • N. Nurcahyani Jurusan Biologi – FMIPA Universitas Lampung – Bandar Lampung
  • H. Busman Jurusan Biologi – FMIPA Universitas Lampung – Bandar Lampung

DOI:

https://doi.org/10.25181/jppt.v16i2.89

Abstract

The aim of this research was to determine the effect of taurine supplementation on the totalcount of leucocyte cells and erythrocyte cells of mice that have been induced to benzo (α)pyrene in vivo. The parameters of this experiment were the total count of leucocyte cells anderythrocyte cells of mice (Mus musculus L.). This experiment was conducted in a completerandomized design by using six treatments, each in five replications. The mice were dividedinto six groups. Group I used as control were not given any treatment. Group II were given0,2 mL of oleum olevarum (olive oil) orally to the end of the experiment. Group III wereinduced to benzo (α) pyrene without being given any taurine. Group IV were given 7,8mg/bw of taurine before being induced to benzo (α) pyrene. Group V were given 7,8 mg/bwof taurine after being induced to benzo (α) pyrene. Group VI were given 15,6 mg/bw oftaurine after being induced to benzo (α) pyrene. Mice of group III, V, and VI were injectedwith 0,5 mL of benzo (α) pyrene solution every day for 10 days on their subcutant tissue forthe purpose of a nodule being formed in this area. It was then continued by giving taurineorally for 15 days. In the final treatment, mice blood was taken to count the leucocyte cellsand erythrocyte cells. The data were analyzed using ANOVA (Analysis of Variance) thencontinued by calculating least significant difference at 5 per cent level of significance. Theresults indicated that taurine had the ability to reduce leucocyte cells into its normalquantity, and was able to increase the number of erythrocyte cells of mice suffered fromleukaemia back to normal.Key words: benzo (α) pyrene, leukaemia, leucocyte, mice , taurine

Downloads

Download data is not yet available.

References

Agra Maysa dkk: Uji Senyawa Taurin Sebagai Antikanker Terhadap Jumlah Sel-Sel Leukosit …

Volume 16, Nomor 2, Mei 2016 75

Saputri, D. N. E., A. P. Dyah, dan N. Abdulgani. 2006. Jumlah Total dan Diferensial Leukosit Mencit (Mus

musculus) pada Evaluasi In Vivo Antikanker Ekstrak Spons Laut Aaptos suberitoides. Skripsi. Fakultas

Matematika dan Ilmu Pengetahuan Alam. Institut Teknologi Sepuluh Nopember. Surabaya.

Schuller-Levis, G. B., E. Park. 2004. Taurine and its chloroamine: modulators of immunity. Neurochemical

Research. 29 (1): 117-126.

Shrivastava, R., S. Srivastava, R. K. Upreti, and U. C. Chaturvedi. 2005. Effects of dengue virus infection on

peripheral blood cells of mice exposed to hexavalent chromium with drinking water. Indian J Med

Res. 122; pp 111-119.

Smeltzer. S. C. dan B. G. Bare. 2002. Buku Ajar Keperawatan Medikal Bedah Brunner dan Suddarth Edisi

, Vol. 1 dan 2. Jakarta: ECG.

Suhardjono, D. 1995. Percobaan Hewan Laboratorium. Yogyakarta: Gajah Mada University Press. hal. 207.

Syarif, A. 1995. Farmakologi dan Terapi. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas

lndonesia edisi-4.

Tambur, Z. 2006. White Blood Cell Differential Count in Rabbits Artificially Infected with Intestinal

Coccidia. J. Protozool. Res, 16, 42-50.

Widmann, F. K. 1995. Tinjauan Klinis Hasil Pemeriksaan Laboratorium Edisi 9. Jakarta: Penerbit Buku

Kedokteran EGC.

Yana, S. 2009. Uji Mutagenisitas Benzo (alfa) piren dengan Metode Mikronukleus pada Sumsum Tulang

Mencit Albino (Mus musculus). Cermin Dunia Kedokteran, Vol 36 no. 1/167.American Cancer Society. 2010. Cancer Statistic 2010. Atlanta, GA: American Cancer Society.

Archer, M. C. 1992. Chemical carcinogenesis. In: Tannock JF, Hill RP, editors. The basic science of

oncology. 2nd ed. New York: Mc Graw-Hill, Inc. p. 102-17.

Baldy, C. M. 2006. Gangguan Sel Darah Putih dan Sel Plasma. Dalam: Price, S. A., L. M. Wilson. 2006.

Patofisiologi Edisi 6. Jakarta: EGC, 271-284.

Birdsall, T. C. 1998. Therapeutic Applications of Taurine. Alternative Medicine Review, 3(2):128-136.

Campbell, N. A., J. B. Reece, L. A. Urry, M. L. Cain, S. A. Wasserman, P. V. Minorsky, dan R. B. Jackson.

Biologi Edisi Kedelapan Jilid 3. Jakarta: Erlangga.

Eilertsen, K., R. Larsen, H. K. Maehre, I. Jensen, dan E. O. Elvevoll. 2012. Anticholesterolemic and

antiatherogenic effects of taurine supplementation is model dependent. Lipoproteins – Role in Health

and Diseases. 269-288.

Fianza, P. I. 2007. Leukemia Limfoblastik Akut, Buku Ajar Ilmu Penyakit Dalam Jilid II. Jakarta: Pusat

Penerbitan Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran, Universitas Indonesia.

Hanahan, D., R. A. Weinberg. 2000. The Hallmark of Cancer. Cell. Vol 100:57-70.

Ismail N. E., R. Suheryanto, S. Kustomo, W. J. B. Harsono. 2005. Efektifitas Extrajoss dalam Memperbaiki

Kinerja Ketahanan Kerja. Badan Penelitian dan Pengembangan Kesehatan Republik Indonesia.

Jakarta.

Kleinsmith, L. J. 2006. Principles of Cancer Biology. Michigan: Pearson Education, Inc.

Learn D. B., V. A. Fried, E. L. Thomas. 1990. Taurine and hypotaurine content of human leukocytes. J

Leukoc Biol, 48:174-182.

Mansjoer, A. 2002. Askarias, dalam: Kapita Selekta Kedokteran Jilid I, Edisi 3. Jakarta: Media Aesculapius

FKUI.

Mosmann, T. 1993. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation

and Citotoxicity Assays. Journal of Immunological Methods, 65, 55-63.

Ripps, H., W. Shen. 2012. Taurin: A Very Essential Amino Acid. Molecular Vision; 18:2673-2686.

Downloads

Published

2017-06-19

How to Cite

Maysa, A., Widiastuti, E. L., Nurcahyani, N., & Busman, H. (2017). Uji Senyawa Taurin Sebagai Antikanker Terhadap Jumlah Sel-Sel Leukosit Dan Sel-Sel Eritrosit Mencit (Mus musculus L.) yang Diinduksi Benzo (Α) Pyren Secara In Vivo. Jurnal Penelitian Pertanian Terapan, 16(2). https://doi.org/10.25181/jppt.v16i2.89

Issue

Vol. 16 No. 2 (2016)

Section

Artikel

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Lisensi Creative Commons