Infection Pathways Beauveria bassiana and Metarhizium anisopliae For Bio-Control of Coleoptera:Oryctes rhinoceros L.

Authors

  • Dyah Nuning Erawati Politeknik Negeri Jember
  • Irma Wardati Program Studi Budidaya Tanaman Perkebunan Politeknik Negeri Jember
  • Suharto Suharto Program Studi Produksi Ilmu Pertanian Universitas Jember
  • Joni Murti Mulyo Aji Program Studi Produksi Ilmu Pertanian Universitas Jember
  • Novita Cholifah Ida Laboratorium Biosain Politeknik Negeri Jember
  • Yeni Suprapti Laboratorium Perlindungan Tanaman Politeknik Negeri Jember

DOI:

https://doi.org/10.25181/jppt.v21i3.2139

Abstract

Recent co-evolutionary studies of insect-pathogens have shown that entomopathogenic fungi have a greater potential for oral toxicity in terms of virulence in the mouth infection of insect pests. Therefore, it is necessary to conduct research on finding the route of infection of                B. bassiana and M. anisopliae through the mouth in the larvae of the rhinoceros beetle                O. rhinoceros. The purpose of this study was to analyze the path of infection of B. bassiana and M. anisopliae in O. rhinoceros larvae through exposure to the entompotagen fungus. The study was conducted at the Plant Protection Laboratory Politeknik Negeri Jember using a single factor Completely Randomized Design (CRD) with B. bassiana and M. anisopliae isolates from Temanggung, Jember and Jombang. Data analysis used Analysis of Variance and further analysis used Least Significant Difference Test at 5% level. The results showed that all isolates were able to infect and cause mortality in the test insects. MJb isolate had the highest effectiveness with the mortality rate of the test insects reaching 100% and the highest spore density with an average of 8.9 x 109 conidia/ml on day 6 after application. The hyphae that were abundant in the abdomen of cadaveric infected with M. anisopliae were different from the hyphae which grew more on the head of insects infected with B. bassiana, confirming previous research on the route of B. bassiana infection through oral toxicity. These results will be useful in providing new information regarding potential pathways of infection of B. bassiana in O. rhinoceros larvae through oral toxicity mechanisms, which until now have been very little reported.   Keywords: entomopathogenic fungi, oral infection, larvae, coleoptera

Downloads

Download data is not yet available.

References

Agastya, I. M. I., Ameliawati, P., dan Fikrinda, W., 2018. Eksplorasi dan identifikasi jamur patogen serangga di rhizosfer lahan kering kabupaten Malang. Jurnal Penelitian Pertanian Terapan, 17(3), pp.13–17. Available at: https://doi.org/10.25181/jppt.v18i1.673.

Aida, I.I., Rasdi, M.Z., Ismail, R., Ismeazilla, M. B., Faisol, M. K., Shakir, M.Z. and Fakriyah, N. A., 2020. Susceptibility and resistance of different host varieties of oil palm and coconut palm towards pest, rhinoceros beetle (Oryctes rhinoceros). Asian Journal of Agriculture and Rural Development, 10(1), pp. 56–67. doi: 10.18488/journal.1005/2020.10.1/1005.1.56.67.

Arsi, Pujiastuti, Y., Kusuma, S. S. H dan Gunawan, B., 2020. Eksplorasi, isolasi dan identifikasi jamur entomopatogen yang menginfeksi serangga hama. Jurnal Proteksi Tanaman Tropis, 1(2), pp. 70–76. Available at: 10.19184/jptt.v1i2.18554.

Biswas, T., Joop, G., and Rafaluk-Mohr, C., 2018. Cross-resistance : a consequence of bi-partite host-parasite coevolution. Insects, 9 (28), pp.1-15. Available at: 10.3390/insects9010028.

Erawati, D. N. and Wardati, I., 2016. Teknologi pengendalian hayati Metarhizium anisopliae dan Beauveria bassiana terhadap hama kumbang kelapa sawit ( Oryctes rhinoceros ). Prosiding seminar Nasional Hasil Penelitian dan Pengabdian Masyarakat (hlm. 1-5). 14 November 2016. Jember : Polije.

Gustianingtyas, M., Herlinda, S., Suwandi, Suparman, Hamidson, H., Hasbi, Setiawan, A., Verawaty, M., Elfita and Arsi, 2020. Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of south sumatra ( Indonesia ) against Spodoptera litura larvae. Biodiversitas, 21(5), pp.1839–1849. Available at: 10.13057/biodiv/d210510.

Herlinda, S., Efendi, R.A., Suharjo, R., Hasbi, Setiawan, A., elfita and Verawaty, M., 2020. New emerging entomopathogenic fungi isolated from soil in south sumatra ( Indonesia ) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas, 21(11), pp.5102–5113. Available at: 10.13057/biodiv/d211115.

Ibrahim, R.A., 2018. Laboratory evaluation of entomopathogenic fungi, commercial formulations, against the rhinoceros beetle , Oryctes agamemnon arabicus ( coleoptera : scarabaeidae ). Egyptian Journal of Biological Pest Control, 27(1), pp. 49-55.

Indriyanti, D.R., Widiyaningrum, P., Haryuni, Slamet, M., and Maretta, Y.A., 2017. Effectiveness of Metarhizium anisopliae and entomopathogenic nematodes to control Oryctes rhinoceros larvae in the rainy season. Pakistan Journal of Biological Science, 20(7), pp. 320-327. Availabel at: 10.3923/pjbs.2017.320.327.

Khun, K. K., Ash, G.J., Stevens M.M, Huwer, R.K., & Wilson, B.A.L, 2021. Transmission of Metarhizium anisopliae and Beauveria bassiana to adults of Kuschelorhynchus macadamiae (Coleoptera : Curculionidae) from infected adults and conidiated cadavers’, Scientific Reports. Nature Publishing Group UK, 11(21), pp. 1–12. Availabel at: 10.1038/s41598-021-81647-0.

Kichaoui, A.Y.E, Asaker, A. A. and El-hindi, M. W., 2017. Isolation , molecular identification and under lab evaluation of the entomopathogenic fungi M . anisopliae and B . bassiana against the red palm weevil R. ferrugineus in Gaza strip. Advances in Microbiology (7), pp. 109-124. Available at: 10.4236/aim.2017.71009.

Mannino, M. C., Huarte-bonnet, C., Davyt-colo, B. & Pedrini, N., 2019. Is the insect cuticle the only entry gate for fungal infection ? insights into alternative modes of action of entomopathogenic fungi. Journal of Fungi, 5(33), pp. 1-9. Available at:10.3390/jof5020033.

Mcguire, A. V and Northfield, T. D., 2020. Tropical occurrence and agricultural importance of Beauveria bassiana and Metarhizium anisopliae. Frontiers in Sustainable Food Systems, 4(6). Availabel at: 10.3389/fsufs.2020.00006.

Pedrini, N., Ortiz-urquiza, A., Huarte-bonnet, C., Zhang, S & Keyhani, N.O., 2013. Targeting of insect epicuticular lipids by the entomopathogenic fungus Beauveria bassiana : hydrocarbon oxidation within the context of a host-pathogen interaction. Frontiers in Microbiology, 4(24), pp. 1–18. doi: 10.3389/fmicb.2013.00024.

Pradipta, A. P., Wagiman, F. X. and Witjaksono, 2020. The potency of collecting larvae of Oryctes rhinoceros L . ( Coleoptera ). Agrivita Journal of Agricultural Science, 42(1), pp. 153–159. Available at: https://doi.org/10.17503/agrivita.v42i1.2489.

Saleem, A. and Ibrahim, R. A., 2019. Assessment of the virulence and proteolytic activity of three native entomopathogenic fungi against the larvae of Oryctes agamemnon (Burmeister) ( Coleoptera : Scarabaeidae. Egyptian Journal of Biological Pest Control, 29 (21). Available at: 10.1186/s41938-019-0120-1.

Suciatmih, Kartika, T. and Yusuf, S., 2015. Jamur entomopatogen dan aktivitas enzim ekstraselulernya. Berita Biologi, 14(2), pp. 131–142.

Valero-jiménez A, C., Faino, L., Veld, D.S., and Van Kan, J.A. L., 2016. Comparative genomics of Beauveria bassiana : uncovering signatures of virulence against mosquitoes. BMC Genomics, 17:986, pp. 1–11. doi: 10.1186/s12864-016-3339-1.

Xiao, G., Ying, S., Zhang, P., Wang, Z., Zhang, S., Xie, X., Shang, Y., Leger R. J. S., Zhoo, G., Wang, C. & Feng, M., 2012. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana. Scientific Reports, 2:483. Available at:10.1038/srep00483.

Downloads

Published

2021-12-31

How to Cite

Erawati, D. N., Wardati, I. ., Suharto, S., Aji, . J. M. M. ., Ida, N. C. ., & Suprapti, Y. . (2021). Infection Pathways Beauveria bassiana and Metarhizium anisopliae For Bio-Control of Coleoptera:Oryctes rhinoceros L. Jurnal Penelitian Pertanian Terapan, 21(3), 220-226. https://doi.org/10.25181/jppt.v21i3.2139

Issue

Section

Artikel