In Vitro Screening Ketahanan Galur Padi (Oryza Sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Keracunan Besi (Fe)

Authors

  • onny Chrisna Pandu Pradana Politeknik Negeri Lampung, Jurusan Budidaya Tanaman Pangan
  • Siti Novridha Andini Politeknik Negeri Lampung, Jurusan Budidaya Tanaman Pangan

DOI:

https://doi.org/10.25181/jppt.v19i3.1527

Keywords:

paddy strain, in vitro, iron toxicity

Abstract

This research aimed to invstigate the response of paddy culture (B7 strain) assembled by Lampung State Polytechnic to the iron toxicity tolerance. The research was done at Plant Tissue Culture Laboratory, Lampung State Polytechnic, from July to September 2019. Treatments were single arranged in a completely randomized design with three replications. The treatment tried was five levels of Fe concentrations (5,6 ppm 28 ppm, 56 ppm, 84 ppm, 112 ppm, and control). Each replication consisted of three culture bottle containing one explant. The homogenity of data was tested using Barlett test. If the assumption were fulfilled then analysis of variance is executed using STATISTIX 10, and then followed by the Honest Significant Difference (HSD) test in 5% alpha for mean separation and RPA analysis. The result of this research showed that the B7 strain has tolerance to iron toxicity until 56 ppm of Fe concentration, it can be concluded from the PAR value of its strain (>0,50). Meanwhile in  84 and 112 ppm of Fe concentration, the RPA value of B7 starin (<0,50), and it is indicate that its strain is sensitive. 

Downloads

Download data is not yet available.

References

Kementrian Pertanian. 2017. Kedaulatan pangan nasional. www.kementan.go.id. Diakses tanggal 15 Februari 2019.

Kementrian Pertanian. 2018. Data kementan selaras dengan data BPS. www. kementan.go.id. Diakses tanggal 15 Februari 2019.

Nugraha Y. dan I. A. Rumanti. 2017. Perakitan Varietas Padi Toleran Keracunan Besi. Iptek Tanaman Pangan 12 (1): 9—24.

Noor, A. Dan Khairuddin. 2013. Keracunan Besi Pada Padi: Aspek Ekologi dan Fisiologi-Agronomi. Seminar Nasional Inovasi Teknologi Pertanian.

Pradana, O. C. P., S. N. Andini, and W. Risa. 2018. In Vitro Sereening of B7 Paddy (Oryza sativa) Strain For pH Stress Tolerance. Prosiding Seminar Nasional Teknologi Pertanian VII. Politeknik Negeri Lampung. Bandar Lampung.

Sankepally, S. S. R. dan B. Singh. 2016. Optimization of regeneration using differential growth regulators in indica rice cultivars. 3 Biotech 6 (19): 1—7.

Suhartini, T. 2010. Pertumbuhan Akar Dua Puluh Genotipe Padi Gogo pada Kahat Fosfor dan Cekaman Alumunium. Berita Biologi 10 (3): 375—383.

Syukur, M., S. Sujiprihati, dan R. Yunianti. 2012. Teknik Pemuliaan Tanaman. Penebar Swadaya. Jakarta.

Umaiyah, E. I. 2016. Respon Fisiologi, Morfologi, dan Anatomi Akar Padi Hawara Bunar Transgenik yang Mengalami Pembungkaman Gen B11 terhadap Cekaman pH Rendah dan Alumunium. (Skripsi) Institut Pertanian Bogor. Bogor.

Wani, S. H., P. A. Sofi, S. S. Gosal, and N. B. Sigh. 2010. In Vitro Sereening of Rice (Oryza sativa L) Callus For Drought Tolerance. Coummunications in Biometry and Crop Science 5 (2): 108—115.

Yusnita. 2010. Perbanyakan In Vitro Tanaman Anggrek. Universitas Lampung. Bandar Lampung. 128 hlm.

Downloads

Published

2019-09-20

How to Cite

Pradana, onny C. P., & Andini, S. N. (2019). In Vitro Screening Ketahanan Galur Padi (Oryza Sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Keracunan Besi (Fe). Jurnal Penelitian Pertanian Terapan, 19(3), 236-243. https://doi.org/10.25181/jppt.v19i3.1527

Issue

Section

Artikel