Prospek Introduksi Regulon nif dari Bakteri Klebsiella pneumoniae ke Dalam Genom Mitokondria Tanaman

Authors

  • Ivan Tjahja Pranata Program Studi Agroteknologi, Fakultas Pertanian, Universitas Kristen Satya Wacana
  • Adminarwati Hulu Program Studi Agroteknologi, Fakultas Pertanian, Universitas Kristen Satya Wacana
  • Yoga Aji Handoko Program Studi Agroteknologi, Fakultas Pertanian, Universitas Kristen Satya Wacana

DOI:

https://doi.org/10.25181/jppt.v18i3.1509

Keywords:

nif regulon, nitrogenase enzym, Klebsiella pneumoniae, mitochondrial genome

Abstract

Use of synthetic fertilizers bring environmental pollution, increase weed growth, reduceatmospheric oxygen levels, decrease the fertility of agricultural land, causing eutrophicationand run-offs, also even damaging coral reefs. Recent biotechnology approaches can beutilized to create a new variety of self-fertilization plants that allows it to supply its ownnitrogen making it possible to overcome nitrogen problems and avoid them. This reviewaims to understand how far genetic engineering approach can be used by the introduction ofnif regulon from Klebsiella penumoniae. The nitrogenase-encoding genes which make up thenif regulon proved can be inserted into the mitochondrial genome as like Allen et al. (2017)did in their research. The western blotting result showed that the research succesfullyintegrated the nif regulon to the genome and expressed their proteins.

Downloads

Download data is not yet available.

References

Allen, R.S., Tilbrook, K., Warden, A.C., Campbell, P.C., Rolland, V., Singh, S.P. & Wood, C.C., 2017.

Expression of 16 nitrogenase proteins within the plant mitochondrial matrix. Frontiers in Plant

Science, 8, article 287. Available at: https://doi.org/10.3389/fpls.2017.00287.

Balk, J. & Pilon, M., 2011. Ancient and essential: the assembly of iron-sulfur clusters in plants. Trends Plant

Science, 16, pp.218-226. Available at: https://doi.org/10.1016/j.tplants.2010.12.006.

Bauman, R.W. 2015. Microbiology with Diseases by Body System. 4

Edition. San Fransisco: Pearson

Benjamin-Cummings.

th

Bhuvaneshwari, K. & Singh, P.K., 2015. Response of nitrogen-fixing water fern Azolla biofertilization to

rice crop. 3 Biotech, 5, pp.523–529. Available at: https://doi.org/10.1007/s13205-014-0251-8.

Bruijn, Frans J. de. 2016. “„Biological Nitrogen Fixation‟ Book Summary.†Advances in Microbiology 6 (6).

Scientific Research Publishing: 407–11. Available at: https://doi.org/10.4236/aim.2016.66040.

Cui, S., Shi, Y., Groffman, P.M., Schlesinger, W.H. & Zhu, Y.G., 2013. Centennial-scale analysis of the

creation and fate of reactive nitrogen in China (1910–2010). Proceedings of the National Academy of

Sciences 110(6), pp.2052–2057. Available at: https://doi.org/10.1073/pnas.1221638110.

Curatti, L. & Rubio, L.M., 2014. Challenges to develop nitrogen fixing cereals by direct nif-gene transfer.

Plant Science, 225, pp.130-137. Available at: https://doi.org/10.1016/J.PLANTSCI.2014.06.003.

Dance, Ian. 2016. Mechanisms of the S/CO/Se interchange reactions at FeMo-Co, the active site cluster of

nitrogenase. Dalton Transactions, 45(36), pp.14285–14300. Available at: https://doi.org/10.1039/

C6DT03159E.

Dixon, R.A. & Postgate, J.R., 1972. Genetic transfer of nitrogen fixation from Klebsiella pneumoniae to

Escherichia coli. Nature, 237, pp.102-103. Available at: http://www.ncbi.nlm.nih.gov/pubmed/

Dixon, R.A & Kahn, D., 2004. Genetic regulation of biological nitrogen fixation. Nature Reviews

Microbiology, 2, pp.621-631. Available at: https://doi.org/10.1038/nrmicro954.

Good, A.G. & Beatty, P.H., 2011. Fertilizing nature: a tragedy of excess in the commons. PLoS Biology, 9,

pp.e1001124. Available at: https://doi.org/10.1371/journal.pbio.1001124.

Hajoeningtijas, O.D. 2012. Mikrobiologi Pertanian. Yogyakarta: Graha Ilmu.

Herridge, D.F., Bergersen, F.J. & Peoples, M.B., 1990. Measurement of nitrogen fixation by soybean in the

field using the ureide and natural

N abundance methods. Plant Physiology, 93, pp.708-716.

Available at: http://www.ncbi.nlm.nih.gov/pubmed/16667527.

Ivleva, N.B., Groat, J., Staub, J.M. & Stephens, M., 2016. Expression of active subunit of nitrogenase via

integration into plant organelle genome. PloS ONE, 11(8), pp.e0160951. Available at:

https://doi.org/10.1371/journal.pone.0160951.

Jimenez-Vicente E, Hernandez JA, Echavarri-Erasun C, Rubio LM. 2015. Biosynthesis of the IronMolybdenum

Cofactor of Nitrogenase. In: de Bruijn FJ. (ed). Biological Nitrogen Fixation.

Hoboken: Wiley-Blackwell Publishers. pp 75-86.

Madigan, M.T., Martinko, J.M., Bender, K.S., Buckley, D.H. & Stahl, D.A.. 2015. Brock Biology of

Microorganisms, 14th Edition. Boston: Pearson.

Martínez, E., Palacios, R., & Sánchez, F., 1987. Nitrogen-fixing nodules induced by Agrobacterium

tumefaciens harboring Rhizobium phaseoli plasmids. Journal of Bacteriology, 169(6), pp.2828-2834.

Available at: http://www.ncbi.nlm.nih.gov/pubmed/3584072.

Mayani, N. & Hapsoh, 2012. Potensi rhizobium dan pupuk urea untuk meningkatkan produksi kedelai

(Glycine max L.) pada lahan bekas sawah. Jurnal Ilmu Pertanian KULTIVAR, 5(2), pp.68-75.

Available at: http://download.portalgaruda.org/article.php?article=51371&val=4106.

Miranda, A., Janssen, G., Hodges L., Peralta, E.G. & Ream, W., 1992. Agrobacterium tumefaciens transfers

extremely long T-DNAs by a unidirectional mechanism. Journal of Bacteriology, 174, pp.2288–

Available at: https://doi.org/10.1128/JB.174.7.2288-2297.1992.

Morett, E. & Segovia, L., 1993. The sigma 54 bacterial enhancer-binding protein family: mechanism of

action and phylogenetic relationship of their functional domains. Journal of Bacteriology, 175,

pp.6067–6074. Available at: http://www.ncbi.nlm.nih.gov/pubmed/8407777.

Mueller, N.D., Gerber, J.S., Johnston, M., Ray, D.K., Ramankutty, N. & Foley, J.A., 2012. Closing yield

gaps through nutrient and water management. Nature, 490, pp.254–257. Available at:

https://doi.org/10.1038/nature11420.

Oldroyd, G.E.D. & Dixon, R., 2014. Biotechnological solutions to the nitrogen problem. Current Opinion in

Biotechnology, 26, pp.19-24. Available at: https://doi.org/10.1016/j.copbio.2013.08.006.

Peters, J.W. & Szilagyi, R.K., 2006. Exploring new frontiers of nitrogenase structure and mechanism.

Current Opinion

in Chemical Biology, 10, pp.101–108. Available at:

https://doi.org/10.1016/J.CBPA. 2006.02.019.

Reddy PM, James EK, Ladha JK. 2002. Nitrogen Fixation in Rice. In: Leigh GJ. (ed) Nitrogen Fixation at

the Millenium. Amsterdam: Elsevier. pp 421-445.

Rosmarkam, A. & N.A. Yuwono. 2002. Ilmu Kesuburan Tanah. Yogyakarta: Kanisius.

Shah, V.K. & Brill, W.J., 1973. Nitrogenase IV. Simple method of purification to homogeneity of

nitrogenase components from Azotobacter vinelandii. Biochimica et Biophysica Acta –

Bioenergetics, 305(2), pp.445–454. Available at: http://www.ncbi.nlm.nih.gov/pubmed/4354875.

Wang, X., Yang, J.G., Chen, L., Wang, J.L., Cheng, Q., Dixon, R., Wang, Y.P., 2013. Using synthetic

biology to distinguish and overcome regulatory and functional barriers related to nitrogen fixation.

PLoS ONE, 8(7), pp.e68677. Available at: https://doi.org/10.1371/journal.pone.0068677.

Downloads

Published

2020-01-10

How to Cite

Pranata, I. T., Hulu, A., & Handoko, Y. A. (2020). Prospek Introduksi Regulon nif dari Bakteri Klebsiella pneumoniae ke Dalam Genom Mitokondria Tanaman. Jurnal Penelitian Pertanian Terapan, 18(3), 215-225. https://doi.org/10.25181/jppt.v18i3.1509

Issue

Section

Artikel