Kandungan Klorofil Planlet Vanili (Vanilla planifolia Andrews) Hasil Seleksi Ketahanan terhadap Cekaman Kekeringan secara In Vitro
DOI:
https://doi.org/10.25181/prosemnas.v0i0.512Abstract
A Study of the content of Chlorophyll a, b and the total on vanilla which was swlwcted in vitro by adding up polyethylene glycol (PEG) 6000 to MS medium at various consentrations of 5%, 10%, 15%, and 20% had been conducted at the Laboratory of Tissue Culture, MIPA faculty, Biology Department, Lampung University, on January to March 2015. At experiment was arranged in a complete random design with six replication. The analysis of variance and BNT test were evaluated in real level of 5%. The calculation and extraction of chlorophyll were conducted by using Harborn (1987) method. This experiment used aceton 80% as solution. The absorbent was measured by using spectrophotometer Shimudzu in 646and 663 wave length.The result showed that the content of chlorophyll a and b and the totalon vanilla planlet were decreased. The content of Chlorophyll a and total of the MS mediumwhich were treated by 5% to 20% concentrations of PEG 6000 was decreased compared tothe control. As for the b chlorophyll, only medium with 15% and 20% threatment weredecreased compared to the control.Key word : Vanilla planifolia Andrews, Chlorophyll, PEG 6000, Drought StressDownloads
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