Aplikasi Kejutan Suhu Terhadap Pembentukan Ikan Patin (Pangasius hypopthalmus) Tetraploid
DOI:
https://doi.org/10.25181/prosemnas.v0i0.478Abstract
This research aims to produce super brood of catfish who has a chromosome structure 4N (tetraploid) which will be used as a basis to generate triploid catfish. The specific objective of research was to determine the proper method to produce tetraploid individual catfish, knowing the character of the growth of catfish tetraploid, and knowing the character of morphometric and meristik catfish tetraploid. The study was conducted in Fisheries Laboratory of Lampung State Polytechnic and planned to be carried through three stages: Formation of catfish tetraploid by applying the method of temperature shock, fish ploidy analysis of treatment outcome, and the tetraploid selection. Special Purpose study is to get the population of catfish that have to be tetraploid or 4N ploidy and produce brood catfish putatively tetraploid. Target outcomes study the third year that the main candidates tetraploid. The results of research conducted showed tetraploid individuals found in all treatments given temperature shock to the level of an individual percentage of catfish tetralploid between 5-30%. The determination of ploidy levels in catfish is done using a calculation of the number nucleolus. Individual catfish nucleolus tetraploid have maximum 5-6 pieces.Keyword: catfish, tetraploid, engineered chromosome, geneticsDownloads
References
Hartono, dkk : Aplikasi kejutan suhu terhadap pembentukan ikan patin (Pangasius hypopthalmus) tetraploid.
Prosiding Seminar Nasional Pengembangan Teknologi Pertanian V Polinela 2016 203
Rustidja S., 1989 Artificial induced breeding and triploidy in the Asian catfish (Clariasbatrachus Linn.).
PhD thesis, Faculty of Graduate Studies, Bogor Agricultural Institute, Bogor, 80 pp.
Sakao S, Fujimoto T, Kimura S, Yamaha E, Arai K., 2006 Drastic mortality in tetraploid induction results
from the elevation of ploidy in masu salmon Oncorhynchus masou. Aquaculture 252: 147-160.
Tave D., 1993 Genetics for fish hatchery managers. Second edition, Van Nostrand Reinhold, New York, pp.
-304.
Thorgaard G. H., 1983 Chromosome set manipulation and sex control in fish. In: Fish physiology. Hoar W.
S., Randall D. J., Donaldson M. (eds), Volume IX B, Academic Press, Inc. London, pp. 405-434.
Thorgaard G. H., Gall G. A. E., 1979 Adult triploid in rainbow trout family genetics. Genetics 93:961-973.
Weber GM, Wiens GD, Welch TJ, Hostuttler MA, Leeds TD., 2013 Comparison of disease resistance
between diploid, induced-triploid, and intercross-triploid rainbow trout including trout selected for
resistance to Flavobacterium psychrophilum. Aquaculture 410–411: 66-71.
Weber GM, Hostuttler MA, Cleveland BM, Leeds TD., 2014 Growth performance comparison of intercross-
triploid, induced triploid, and diploid rainbow trout. Aquaculture 433: 85-93.
Weber GM, Hostuttler MA, Semmens KJ, Beers BA., 2015 Induction and viability of tetraploids in brook
trout (Salvelinus fontinalis). Canadian Journal of Fisheries and Aquatic Sciences, 72(10): 1443-1449.
Zhang X, Asami T, Onozato H., 2007 Polypolar spindle formation during first cell cycle in rainbow
trout Oncorhynchus mykiss embryos after heat-shock treatment. Fish. Sci. 73: 1325-1331.
Zhang Z, Chen J, Li L, Tao M, Zhang C, Qin Q, Xiao J, Liu Y, Liu S. 2014 Research advances in animal
distant hybridization. Sci. China Life Sci. 57: 889-902
Zohar Y., 1989 Fish reproduction: its physiology and artificial manipulation Creating Bibliography. In: Fish
culture in warm water systems: problems and trends. Shilo M., Sarig S. (eds), CRC Press, Inc. Boca
Raton, Florida, pp. 65-120.Hartono, dkk : Aplikasi kejutan suhu terhadap pembentukan ikan patin (Pangasius hypopthalmus) tetraploid.
Prosiding Seminar Nasional Pengembangan Teknologi Pertanian V Polinela 2016
El Gamal A-RA, Davis KB, Jenkins JA, Torrans LE., 1999 Induction of triploidy and tetraploidy in Nile
tilapia, Oreochromis niloticus (L). J. World Aquacult. Soc. 30: 269-275.
Gil, Hyun Woo; Kong, Hee Jeong; An, Cheul Min; Kim, Bong-seok; Lim, Sang-gu., 2016 Cytogenetic study
of diploid and induced tetraploid in Korean rose bitterling, Rhodeus uyekii. SpringerPlus 5.1 (Feb
: 1-10.
Goudie CA, Simco BA, Davis KB, Liu Q., 1995 Production of gynogenetic and polyploid catfish by
pressure-induced chromosome set manipulation. Aquaculture 133: 185-198.
Hartono D. P., Febriani D., Marlina E., 2009 The temperature influence on the formation of individual cold
shock triploid catfish (Pangasius sp). Proceedings of the National Seminar on Appropriate Technology
Agroindustri, Lampung State Polytechnic, Lampung, 55 p.
Hartono, D.P., P. Witoko., N. Pubosari., 2016. The effect of heat shock on the tetraploidy of catfish,
Pangasius hypopthalmus. AACL Bioflux, 2016, Volume 9, Issue 3.
Ihssen P. E., McKay L. R., McMillan I., Phillips R. B., 1990 Ploidy manipulation and gynogenesis in fishes:
cytogenetic and fisheries applications. Transaction of the American Fisheries Society 119:698-717.
Jun D, Chang Y, Wang Z, Song J., 2007 Polyploidy induction by hydrostatic pressure shock and embryo
development of sea cucumber, Apostichopus japonicus. Chin J Oceanol Limnol 25:184–190.
Lagler K. F., Bardach J. E., Miller R. R., Passino D. R. M., 1977 Ichthyology. John Willey and Sons Inc,
New York-London, 506 pp.
Lebeda, I and M. Flaishans., 2015 Technical note: Production of tetraploid sturgeons. American Society of
Animal Science 93:3759–3764.
Luo K, Xiao J, Liu S, Wang J, He W, Hu J, Qin Q, Zhang C, Tao M, Liu Y., 2011 Massive production of all-
female diploids and triploids in the crucian carp. Int. J. Biol. Sci. 7: 487-495.
Malison JA, Kayes TB, Held JA, Barry TP, Amundson CH., 1993 Manipulation of ploidy in yellow perch
(Perca flavescens) by heat shock, hydrostatic pressure shock, and spermatozoa inactivation.
Aquaculture 110: 229-242.
Malison JA, Garcia-Abiado MAR., 1996 Sex control and ploidy manipulations in yellow perch (Perca
flavescens) and walleye (Stizostedion vitreum) J. Appl. Ichthyol. 12: 189-194.
Myers JM. 1986 Tetraploid induction in Oreochromis spp. Aquaculture 57: 281-287.
Myers JM, Hershberger WK., 1991 Early growth and survival of heat-shocked and tetraploid-derived triploid
rainbow trout, (Oncorhynchus mykiss) Aquaculture 96: 97-107.
Hartono, dkk : Aplikasi kejutan suhu terhadap pembentukan ikan patin (Pangasius hypopthalmus) tetraploid.
Prosiding Seminar Nasional Pengembangan Teknologi Pertanian V Polinela 2016
El Gamal A-RA, Davis KB, Jenkins JA, Torrans LE., 1999 Induction of triploidy and tetraploidy in Nile
tilapia, Oreochromis niloticus (L). J. World Aquacult. Soc. 30: 269-275.
Gil, Hyun Woo; Kong, Hee Jeong; An, Cheul Min; Kim, Bong-seok; Lim, Sang-gu., 2016 Cytogenetic study
of diploid and induced tetraploid in Korean rose bitterling, Rhodeus uyekii. SpringerPlus 5.1 (Feb
: 1-10.
Goudie CA, Simco BA, Davis KB, Liu Q., 1995 Production of gynogenetic and polyploid catfish by
pressure-induced chromosome set manipulation. Aquaculture 133: 185-198.
Hartono D. P., Febriani D., Marlina E., 2009 The temperature influence on the formation of individual cold
shock triploid catfish (Pangasius sp). Proceedings of the National Seminar on Appropriate Technology
Agroindustri, Lampung State Polytechnic, Lampung, 55 p.
Hartono, D.P., P. Witoko., N. Pubosari., 2016. The effect of heat shock on the tetraploidy of catfish,
Pangasius hypopthalmus. AACL Bioflux, 2016, Volume 9, Issue 3.
Ihssen P. E., McKay L. R., McMillan I., Phillips R. B., 1990 Ploidy manipulation and gynogenesis in fishes:
cytogenetic and fisheries applications. Transaction of the American Fisheries Society 119:698-717.
Jun D, Chang Y, Wang Z, Song J., 2007 Polyploidy induction by hydrostatic pressure shock and embryo
development of sea cucumber, Apostichopus japonicus. Chin J Oceanol Limnol 25:184–190.
Lagler K. F., Bardach J. E., Miller R. R., Passino D. R. M., 1977 Ichthyology. John Willey and Sons Inc,
New York-London, 506 pp.
Lebeda, I and M. Flaishans., 2015 Technical note: Production of tetraploid sturgeons. American Society of
Animal Science 93:3759–3764.
Luo K, Xiao J, Liu S, Wang J, He W, Hu J, Qin Q, Zhang C, Tao M, Liu Y., 2011 Massive production of all-
female diploids and triploids in the crucian carp. Int. J. Biol. Sci. 7: 487-495.
Malison JA, Kayes TB, Held JA, Barry TP, Amundson CH., 1993 Manipulation of ploidy in yellow perch
(Perca flavescens) by heat shock, hydrostatic pressure shock, and spermatozoa inactivation.
Aquaculture 110: 229-242.
Malison JA, Garcia-Abiado MAR., 1996 Sex control and ploidy manipulations in yellow perch (Perca
flavescens) and walleye (Stizostedion vitreum) J. Appl. Ichthyol. 12: 189-194.
Myers JM. 1986 Tetraploid induction in Oreochromis spp. Aquaculture 57: 281-287.
Myers JM, Hershberger WK., 1991 Early growth and survival of heat-shocked and tetraploid-derived triploid
rainbow trout, (Oncorhynchus mykiss) Aquaculture 96: 97-107.
Hartono, dkk : Aplikasi kejutan suhu terhadap pembentukan ikan patin (Pangasius hypopthalmus) tetraploid.
Prosiding Seminar Nasional Pengembangan Teknologi Pertanian V Polinela 2016
El Gamal A-RA, Davis KB, Jenkins JA, Torrans LE., 1999 Induction of triploidy and tetraploidy in Nile
tilapia, Oreochromis niloticus (L). J. World Aquacult. Soc. 30: 269-275.
Gil, Hyun Woo; Kong, Hee Jeong; An, Cheul Min; Kim, Bong-seok; Lim, Sang-gu., 2016 Cytogenetic study
of diploid and induced tetraploid in Korean rose bitterling, Rhodeus uyekii. SpringerPlus 5.1 (Feb
: 1-10.
Goudie CA, Simco BA, Davis KB, Liu Q., 1995 Production of gynogenetic and polyploid catfish by
pressure-induced chromosome set manipulation. Aquaculture 133: 185-198.
Hartono D. P., Febriani D., Marlina E., 2009 The temperature influence on the formation of individual cold
shock triploid catfish (Pangasius sp). Proceedings of the National Seminar on Appropriate Technology
Agroindustri, Lampung State Polytechnic, Lampung, 55 p.
Hartono, D.P., P. Witoko., N. Pubosari., 2016. The effect of heat shock on the tetraploidy of catfish,
Pangasius hypopthalmus. AACL Bioflux, 2016, Volume 9, Issue 3.
Ihssen P. E., McKay L. R., McMillan I., Phillips R. B., 1990 Ploidy manipulation and gynogenesis in fishes:
cytogenetic and fisheries applications. Transaction of the American Fisheries Society 119:698-717.
Jun D, Chang Y, Wang Z, Song J., 2007 Polyploidy induction by hydrostatic pressure shock and embryo
development of sea cucumber, Apostichopus japonicus. Chin J Oceanol Limnol 25:184–190.
Lagler K. F., Bardach J. E., Miller R. R., Passino D. R. M., 1977 Ichthyology. John Willey and Sons Inc,
New York-London, 506 pp.
Lebeda, I and M. Flaishans., 2015 Technical note: Production of tetraploid sturgeons. American Society of
Animal Science 93:3759–3764.
Luo K, Xiao J, Liu S, Wang J, He W, Hu J, Qin Q, Zhang C, Tao M, Liu Y., 2011 Massive production of all-
female diploids and triploids in the crucian carp. Int. J. Biol. Sci. 7: 487-495.
Malison JA, Kayes TB, Held JA, Barry TP, Amundson CH., 1993 Manipulation of ploidy in yellow perch
(Perca flavescens) by heat shock, hydrostatic pressure shock, and spermatozoa inactivation.
Aquaculture 110: 229-242.
Malison JA, Garcia-Abiado MAR., 1996 Sex control and ploidy manipulations in yellow perch (Perca
flavescens) and walleye (Stizostedion vitreum) J. Appl. Ichthyol. 12: 189-194.
Myers JM. 1986 Tetraploid induction in Oreochromis spp. Aquaculture 57: 281-287.
Myers JM, Hershberger WK., 1991 Early growth and survival of heat-shocked and tetraploid-derived triploid
rainbow trout, (Oncorhynchus mykiss) Aquaculture 96: 97-107.Beaumont A. R., 1994 Genetics and evolution of aquatic organisms. Chapman & Hall, London, pp. 467-485.
Bidwell C. A., Chrisman C. L., Libey G. S., 1985 Polyploidy induced by heat shock in channel catfish.
Aquaculture 51:25-32.
Carman O., 1992 Chromosome set manipulation in some warm-water fish. PhD Thesis, Tokyo University of
Fisheries, Tokyo, 131 pp.
Chao N. H., Hsu H. W., Hsu H. Y., Liang W. H., Liao I. C., 1993 Studies on methods of triploidy percentage
analysis. TML Conference Proceedings 3:203-210.
Chourrout D, Chevassus B, Krieg F, Happe A, Burger G, Renard P., 1986 Production of second generation
triploid and tetraploid rainbow trout by mating tetraploid males and diploid females — potential of
tetraploid fish. Theor. Appl. Genet. 72: 193-206.
Chourrout D. 1984 Pressure-induced retention of second polar body and suppression of first cleavage in
rainbow trout: Production of all-triploids, all-tetraploids, and heterozygous and homozygous diploid
gynogenetics. Aquaculture 36: 111-126.
Corely-Smith G. E., Lim C. J., Brandhorst B. P., 1996 Production of androgenetic zebrafish (Danio rerio).
Genetics 142:1265-1276.
Effendi M. I., 1979. Fisheries biological method. Dewi Sri Foundation, Bogor, 112 pp.
