Pengaruh Perlakuan Awal Basa dan Hidrolisis Asam terhadap Kadar Gula Reduksi Ampas Tebu

Authors

  • Sutikno Sutikno Jurusan Teknologi Hasil Pertanian Universitas Lampung
  • Novita Sari Jurusan Teknologi Hasil Pertanian Universitas Lampung
  • Marniza Marniza Jurusan Teknologi Hasil Pertanian Universitas Lampung

DOI:

https://doi.org/10.25181/prosemnas.v0i0.406

Abstract

Bagasse, solid by product of sugar cane industries, contains high lignocellulose consisting of 46.3% cellulose, 23.0% hemicellulose and 19.7% lignin. The bagasse can be converted into bioethanol after pretreatment with base and acid and then fermenting with microbes. The objective of this study was to find out the effects of sodium hydroxide pretreatment and sulfuric acid hydrolysis on bagasse reduced sugar. In this study, there were 2 treatments with 3 replications. The first treatment was submerssion bagasse into 0.50 M NaOH solution at a temperature of 121oC for 15 minutes. The second treatment was concentrations of sulphuric acid (H2SO4) that consisted of 5 levels, i.e. 0 M, 0.05 M, 0.10 M, 0.20 M, and 0.30 M. One and an half grams of dried and ground bagasse was put into 100 mL Erlemeyer flash and then added with 30 mL 1.0 M NaOH solution. The flash was heated at a temperature of 121oC for 15 minutes. After filtering, the residue was hydrolyzed with H2SO4 solution. The residue as well as 1.5 g dried and ground bagasse without pretreatment with NaOH was hydrolyzed with 15 mL H2SO4 at concentrations of 0, 0.05, 0.10, 0.20, and 0.30 M at a temperature of 121oC for 15 minutes. Filtrates of the solutions were taken to analyze their reduced sugar content. Reduced sugar of the bagasse samples ranged from 0.05 to 4.20 mg/100 mL. The highest reduced sugar (4.20 ml/mL) was yielded when bagasse was directly, without treating with NaOH, hydrolyzed with 0.05 M H2SO4 at a temperature of 121oC for 15 minutes. Keywords : Bagasse, lignocellulose, sulphuric acid, reducing sugar, Sodium hydroxide.

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References

Sutikno, Novita Sari, Marniza: Pengaruh Perlakuan Awal Basa dan Hidrolisis Asam...

Prosiding Seminar Nasional Pengembangan Teknologi Pertanian Polinela 2014

Sutikno., Hidayati, S., Nawansih, O., Nurainy, F., Rizal, S., Marniza., dan Arion, R. 2010. Tingkat

Degradasi Lignin Bagas Tebu Akibat Perlakuan Basa Pada Berbagai Kondisi.

Disampaikan Dalam Seminar Nasional Teknologi Tepat Guna di Politeknik Negeri

Lampung Pada Bulan April. http://blog.unila.ac.id/sutiknounila/category/research-

activities. Diakses pada tanggal 26 Juni. 2010.

Taherzadeh, M.J., and Karimi, K. 2007. Acid-based hydrolysis processes for ethanol from

lignocellulosic materials : A review. BioResources 2 (3) : 472-499. Achmadi. 1990. Kimia Kayu. Bahan Pengajaran Universitas Ilmu Hayati.Institut Pertanian

Bogor. 120 hlm.

Anonim. 2009. Biomasa limbah agroindustri. http: //

elearning.unej.ac.id/courses/PNU1705/document/babIklpswt.docNU1705. Diakses pada

tanggal 7 Agustus 2012.

Badger, P.C. 2002. Ethanol from cellulose : A general review. P 17-21 In : J. Janick and A.

Whipkey (eds) Trenin new crop and new uses. ASHS Press, Alexandria, VA., USA.

Datta, R. 1981. Acidogenic fermentation of linocellulose acid yield and convertion of components.

Biotechno. Dioeng 23. Hlm 2167-2170.

Faisal, A. 2008. Produk si xilitol oleh khamir penghasil enzim xylose reductase dari hidrolisat

ampas tebu. Skripsi. UI. Depok.

Gomez, L.D., Steel-King, C.G., and Mc Queen-Mason, J. 2008. Sustainable liquid biofuels from

biomass : the writing’s on the wall. New Phytologist 178 : 473-485.

Heradewi. 2007. Isolasi Lignin Dari Lindi Hitam Proses Pemasakan Organosolv Serat Tandan

Kosong Kelapa Sawit (TKKS). Skripsi. IPB. Bogor.

Mussatto, S.I., and Roberto, I.C., 2004. Alternatives for detoxification of dilute-acid lignocellulosic

hydrolyzates for use in fermentative process: a review. Bioresource Technology, 93, 1-10.

Palmquist, E and Hahn-Hageral, B. 2000. A Review: Fermentation of lignocellulosic hydrolysate

II, Inhibitor and mechanism of inhibitor; Bioresource Technol.

Putri, F. Y. 2010. Pengaruh Konsentrasi NaOH dan Lama Perendeman TKKS (Elaeis guinensis

JACQ) Terhadap Kadar Hemiselulosa, Selulosa, dan Lignin Untuk Produksi Biobutanol.

Skripsi. Teknologi Hasil Pertanian. Universitas Lampung. 96 hlm.

Samsuri, M., Gozam, M., Mardias, R., Baiquni, M., Hermansyah, H., Wijanarko, A., Prasetya, B,

dan Nasikin, M. 2007. Pemanfaatan sellulosa bagas untuk produksi ethanol melalui

sakarifikasi dan fermentasi serentak dengan enzim xilanase. Makara Teknologi. 11(1): 17-

Septiyani, R. 2011. Pengaruh Konsentrasi Dan Waktu Inkubasi Enzim Selulase Terhadap Kadar

Gula Eduksi Ampas Tebu. Skripsi. Teknologi Hasil Pertanian. Universitas Lampung. 53

hlm.

Sudarmadji, S., Bambang, H., dan Suhardi. 1984. Prosedur Analisa untuk Bahan Makanan dan

Pertanian edisi ketiga. Liberty. Yogyakarta.

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2017-10-30

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