Aktivitas Antibakteri Minuman Sinbiotik Cincau Hijau dengan Penambahan Sari Buah Terhadap Staphylococcus aureus dan Eschericia coli Selama Penyimpanan

Abstract

 Synbiotic beverage of Premna oblongifolia Merr extract has the ability to suppress the growth of pathogenic bacteria that cause diarrhea, such as Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Salmonella sp. This study was conducted to determine the antibacterial activity of synbiotic beverage of Premna oblongifolia Merr extract with the addition of guava or pineapple fruit juice against the test bacteria, Staphylococcus aureus, and Escherichia coli during storage time. Products were stored for 28 days in the refrigerator (4°C ± 2°C). Antibacterial activity was assayed by the agar diffusion method.  The average of the data was presented on tables and diagrams, then analyzed descriptively.  The results showed that the inhibition of Escherichia coli growth was greater than Staphylococcus aureus. Synbiotic beverage of Premna oblongifolia Merr extract with addition of guava juice during storage produce a log of total BAL range from 10,90-10,27 Log colonies/ml, equivalent to 8,0x10 10 10 -1,9x10  CFU/ml, total acid 0,50-0.90%, and pH  3,61-3,84, while the addition of pineapple juice has resulted in a log of total BAL range from 10.82-10.11 Log colonies/ml, equivalent to 6,8x10 10 10 -1,3x10  CFU/ml, total acid 0,54- 0,95%, and pH 3,52-3,76.Keywords: synbiotic beverage, Premna oblongifolia Merr, fruit juice; antibacterial activity