In Vitro Screening Ketahanan Galur Padi (Oryza sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Cekaman pH Lingkungan Tumbuh

Abstract

This research aimed to invstigate the response of paddy culture (B7 strain) assembled by Lampung State Polytechnic to the pH stress of environment growth. The research was done at Plant Tissue Culture Laboratory, Lampung State Polytechnic, from May to August 2018. Treatments were single arranged in a completely randomized design with three replications. The treatment tried was five levels of culture media pH (3.5; 4; 4.5; 5; and 5.8). Each replication consisted of three culture bottle containing one explant. The homogenity of data was tested using Barlett test. If the assumption were fulfilled then analysis of variance is executed using STATISTIX 10, and then followed by the Honest Significant Difference (HSD) test in 5% alpha for mean separation. The result of this research showed that the B7 strain gives the same growth response at various pH levels tested, except at pH 3.5. Thus it can be assumed that the B7 strain has tolerance characteristic to low pH until pH 4. Furthermore, further testing in the field is needed to investigate the yield potential of B7 strain if planted at low pH. Keywords: paddy strain, in vitro, pH media