In vitro regeneration of porang (Amorphophallus muelleri Blume) at several concentrations of BAP (benzyl amino purine)

Abstrak

There is problem about propagating porang by seed that caused it is not always available. Planting by bulbs, bulbil, or leaf cuttings need longer times caused by dormancy problems. Micropropagation by tissue culture is an alternative method that can be choose to fix the problem. This research is aim to obtain the best concentration of BAP to regenerate ‘Madiun 1’ variety of porang plants by shoots (organogenesis) formation. The research was carried out using complete randomized design with 7 treatments concentrations of BAP, that was: P1 (0 mg l-1 BAP), P2 (1 mg l-1 BAP), P3 (2 mg l-1 BAP), P4 (3 mg l-1 BAP), P5 (4 mg l-1 BAP), P6 (5 mg l-1 BAP), and P7 (6 mg l-1 BAP). Each treatment was consisted of 5 replications. Observation were made on number of budding, number of shoots, and shoot height. The data were analyzed using ANOVA, and the differences between treatments were tested using the LSD test at the 5% level. The results revealed that 2 mg l-1 (P3) was the best concentration of BAP to be added to the media to regenerate katak porang tubers of Porang Madiun 1 by in vitro.