In-vitro culture techniques of mini tomato for genomic study preparation

Abstrak

Mini tomato cv. Micro-Tom is a plant favored by researchers because it has a shortlife cycle and small in size so that it becomes a practical plant and is suitable for representingresearch on tomato plants or other plants of the Solanaceae family. Unlike ordinary tomatoplants which are large, take up space to grow, and sometimes have a long life cycle, makingordinary tomato plants a plant that is not so easy to handle. For that reason, the mini tomato isnow described as a laboratory tomato. The aim of research is prepared mini tomato forgenomic study. The research was conducted at Politeknik Negeri Lampung Tissue CultureLaboratory from April to September 2020. The material used for this research was mini tomatoseeds. The explants used for the multiplication in this study were the cotyledons. These studiesused macro elements, microelements, tomato vitamins, CaCl2, and FeSO4. EDTA to makeMurashige and Skoog (MS) media, co-cultivation media, and callus induction media, shootinduction media, and root induction media for generated tomato plants. We successfullydeveloped a mini tomato until final root induction. The result of these studies will use forgenomic study (next research).