Micropropagation of Java Cardamom (Amomum compactum)

Authors

  • Lukita Devy Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Henti Rosdayanti Research Center for Research Center for Plant Conservation, Botanic Gardens and Forestry, National Research and Innovation Agency, National Research and Innovation Agency (BRIN), Bogor, Indonesia
  • Hayat Khoiriyah Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Tati Sukarnih irectorate of Laboratory Management, Research Facilities, and Science and Technology Park, South Tangerang, Indonesia.
  • Karyanti Karyanti Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Winda Nawfetrias Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Sasanti Widiarsih Research Center for Radiation Process Technology, National Research and Innovation Agency (BRIN), Jakarta, Indonesia.
  • Cheppy Syukur Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Adi Setiadi Research Center for Horticultural and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
  • Yuda Purwana Roswanjaya Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia.

DOI:

https://doi.org/10.25181/icoaas.v3i3.2866

Abstract

Java cardamom (Amomum compactum) is hardly propagated with rhizome without the mother plant.  In vitro culture could overcome the problem through mass propagation for seedling production or other purposes such as genetic material for mutation breeding. The aim of the research was to generate protocol of establishing Java cardamom micropropagation.  This research consisted of 4 aspects i.e. shoot induction of mother plant (without Plant Growth Regulator (PGR) and using PGR BAP (6-benzylaminopurine) 1000 ppm), explant origin selection (main stem, rhizome bud height >3 cm, rhizome bud height ≤3 cm and lateral rhizome), sterilization procedure establishment (4 methods differ in the use of  detergent, HgCl2, Alcohol, NaOCl, Ethanol, Iodine and soaking time in fungicide and bactericide) and shoot multiplication (MS 0, MS 0 + BAP 1 ppm and MS 0 + BAP 1 ppm + NAA 1 ppm). Result showed the application of 1000 ppm BAP to mature plant could induce shoot emergence.  The best explant source was rhizome bud that smaller or equal to 3 cm.  The highest survival rate (71%) was recorded when explants disinfected with 70% alcohol for 30 seconds and 0.1 % mercuric chloride for 5 minutes.  Java cardamom in vitro culture showed highest shoot multiplication rate in MS 0 + BAP 1 ppm medium (multiplier of 10 shoots/explant in 18 weeks).  Keywords: explant, in vitro propagation, Plant Growth Regulator, rhizome, shoot multiplication

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Published

2023-03-14