Pengaruh Beberapa Komposisi Media Tumbuh in vitro terhadap Pertumbuhan Plantlet Kentang Varietas Granola

Asih . K. Karjadi, Nurmalita Waluyo

Sari


The use of in vitro techniques for vegetative propagation purposes is the most advanced area in tissue culture techniques. Propagation of potato plants (Solanum tuberosum L) in vitro has several advantages that are disease-free, rapidly produce in large quantities and can be done throughout the year. The aim of the study was to look for growing media compositions for Granola plantlets that could increase the growth of potato cuttings in vitro in a relatively short time. The research was conducted at Tissue Culture Laboratory of Indonesian Vegetable Research Institute in April until  September 2015. The treatment is A = growth media composition A1 = MS + coconut water 100 ml / l + sugar 40 g / l., PH 5.7; A2 = MS + Myo inositol 100 mg / l + folicacid 1 mg / l + CaP 2 mg / l + putrecine HCl 10 mg / l + sucrose 30 g / l, pH 5.7; A3 = MS + Myo inositol 100 mg / l + GA3 0.1 mg / l + sucrose 25 g / l, pH 5.7; A4 = MS + GA3 0.15 mg / l + sucrose 25 g / l, pH 5.7; B = growing media density, M1 = semi-solid, agar 3 g / l; M2 = solid medium for 6 g / l. The design used Randomized Block Design, each treatment was repeated 10 times and planted 2 explant each consisting of 2 nodus in reaction tube 25 x 200 mm with 10 ml media volume. Observation result of growth, statistic analysis between treatment solidity and media composition there is no interaction. Visually the growth of Granola plantlet varieties on different solid and solid media, plantlet growth on A3 medium (MS + Myo inositol 100 mg / l + GA3 0.1 mg / l + sucrose 25 g / l, pH 5.7) was the best when compared with other media compositions.

 

Keywords: potato (Solanum tuberosum L), plantlet, MS medium, solid, semi solid.


Teks Lengkap:

Halaman 37-42

Referensi


Armini; G.A Wattimena , L. Winata. Perbanyakan tanaman dalam bioteknologi Tanaman I. Wattimena et al (eds). PAU Bioteknologi IPB. Dirjen Dikti. DEpt P&K, hal 12 – 18.

Badoni A and Chamka J.S. 2010. In vitro sterilization protocol for micropropagation of solanum tuberosum cv. Kufri Himalini . Acad.Arena 2 (40p 24- 27.

Facioli G and Colalongo M.C. 2002. Eradications of potato virus Y and potato leafroll virus by chemoteraphy of infected potato stem cuttings. Phytopathol. Mediter. 41; 76-78.

George, E.F; Hall .M.A. and De Klerk .G.J. 2008.The component of plant tissue culture medium I. Macro and micro nutrients in (eds) George E. F.et al . Plant propagation by tissue culture the back ground Vol I 3rd ed. Spriner Netherlands, pp 274 – 338.

Gunawan, L.W. 1995. Teknik kultur in vitro dalam hortikultura. Penebar Swadaya.

Gunawan . L. 1987. Teknik kutur jaringan . PAU. IPB Bogor 252 pp.

Moreira, Dias; R.V.Molina; J.L. Guardiola and Agarcia Luis. 2000. Direct and indirect shoot organogenic pathways in epicotyls cuttings of troyer citrange differ in ZPT requirements and in their respons of light. Ann Bot. 85 ; 103 – 110.

Murashige .T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Planta. 15; 473 - 497.

Naik, P.S. and Chandra P. 1993. Use of tissue culture technique in crop improvement with special reference to potato- CPRI Shinla.

Thorpe, 19811. Plant tissue culture. Methods and application in Agric. Acad. Inc. N.Y.

Shen Y; M.E. Kane and J.Chen. 2008. Effectsof genotype explants source and plant growth regulators on indirect shoot organogenesis in dieffenbachia cultuvars in vitro cell dev. Biol Plant. 44 ; 282 -288.

Wattimena, G.A. 1986. Kultur jaringan tanmanan kentang. Makalah dalam training course on potato seed technology. Dir . Bina Prod. Hort – FAO. 27 Oct – 8 Nov. 1986.

Wattimena G.A.; Nurhayati M; Armini N.M.; Purwito A Efendi D; Purwoko B. S. dan Khumaida . N 2011. Bioteknologi dalam pemuliaan Tanaman. IPB. Press. Bogor, p 45 – 62.




DOI: http://dx.doi.org/10.25181/prosemnas.v0i0.702

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