Pengaruh Penambahan BAP Dan GA3 Terhadap Pertumbuhan Tunas in Vitro Tanaman Kentang (Solanum Tuberosum L)

Asih K. Karjadi, Nurmalita Waluyo

Sari


Potato plant (Solanum tuberosum L) is included in the family solanaceae and is one of the prioritized vegetable crops. The study was carried out in a tissue culture laboratory at the Vegetable Crops Research Institute in April until September 2014, the purpose of the study to see the effect of increasing the growth hormone of BAP and GA3 on the growth of in vitro buds of potato plants. The treatment was in vitro cultivation of varieties of Granola on MSMA MS + MS vitamin + supplement (coconut water 100 ml / l + Calcium panthotenate 2 mg / l + Myo inositol 100 mg / l + sukrose 30 g / l + to 6-6.5 g / L, pH 5.7), as the growing media treatment plus BAP (0; 0.5; 1 mg / l) and GA3 (0; 0.05; 0.15; 0.20; 0.25 mg / l). The design used, Randomized Block Design with 10 replications. The results of plant growth observation are (1) the growth of explant shoots is always faster than the nodus, (2) the addition of BAP in MS media increases the number of books and plant height, (3) the result of statistical analysis of GA3 addition in MS medium has no significant effect. Visually the addition of GA3 with a concentration of 0 - 0.15 mg / l is the best for the growth of Granola potato plant varieties in vitro.

 

Keywords: Potato (Solanum tuberosum L), In vitro Plant, BAP, GA3, MS


Teks Lengkap:

Halaman 9-14

Referensi


Armini; G.A Wattimena , L. Winata. Perbanyakan tanaman dalam bioteknologi Tanaman. PAU Bioteknologi IPB. Dirjen Dikti. Dept P&K, hal 12 – 18.

Facioli G and Colalongo M.C. 2002. Eradications Of Potato Virus Y And Potato Leafroll Virus By Chemoteraphy Of Infected Potato Stem Cuttings. Phytopathol. Mediter. 41; 76-78.

Goodwin, P.B. 1980. Methods For Rapid Propagation of Potatoes. Paper presented in symposium at potato production in the tropics. Bandung.

Gunawan, L.W. 1987. Teknik Kultur Jaringan Tumbuhan. Lab. Kultur Jaringan Tumbuhan . PAU Bioteknologi IPB. Ditjen Pendidikan Tinggi P& K, 1995. Teknik kultur in vitro dalam hortikultura . Penebar Swadaya.

Karjadi, A.K. 1996. Perbaikan Sistim Pembibitan Kentang Melalui Teknik Kultur Jaringan Dan Teknik Perbanyakan Cepat. Balai Penel. Tan. Sayuran. Puslithort. Badan Litbang Pertanian. JICA – ATA 524 , 35 pp.

Karta , K.K. 1981. Meristem Culture And Cryoperservation , Methods And Application . In Thorpe (ed). Plant tissue culture methods and application in Agric. Acad. Press. Inc. N.Y.

Mellor, F.C. and Stance Smith, 1967. Eradication Of Virus X , Thermotheraphy, Phytophatology 57 ; 674 – 678.

Murashige .T and Skoog F, 1962. A Revised Medium For Rapid Growth And Bioassays With Tobacco Tissue Cultures. Physiol. Planta. 15; 473 - 497.

Naik, P.S. and Chandra P. 1993. Use Of Tissue Culture Technique In Crop Improvement

With Special Reference To Potato- CPRI Shinla.

Shen Y; M.E. Kane and J.Chen. 2008. Effectsof Genotype Explants Source And Plant Growth Regulators On Indirect Shoot Organogenesis In Dieffenbachia Cultuvars In Vitro Cell Dev. Biol Plant. 44 ; 282-288.

Wattimena G, 1986. Kultur Jaringan Tanaman Kentang, Makalah Dalam Training Course On Potato Seed Technology. Dir. Bina Prod Hort. FAO, 27 Oct – 8 Nov 1986.

Westcott, R; J.G.G. Henshew and W.N. Roca. 1977. Tissue Culture Storage And Potao Germplasm Culture, Initiation And Plant Regeneration . Plant Sci. Letters 9 ; 309 – 315.

Quak, F 1961. Treatment and Substance Virus Multiplication In Meristem Culture To Obtain Virus Free Plant. Ad. Hort. Sci. 141 – 144.




DOI: http://dx.doi.org/10.25181/prosemnas.v0i0.698

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