In-vitro culture techniques of mini tomato for genomic study preparation

Abstrak

Mini tomato cv. Micro-Tom is a plant favored by researchers because it has a short
life cycle and small in size so that it becomes a practical plant and is suitable for representing
research on tomato plants or other plants of the Solanaceae family. Unlike ordinary tomato
plants which are large, take up space to grow, and sometimes have a long life cycle, making
ordinary tomato plants a plant that is not so easy to handle. For that reason, the mini tomato is
now described as a laboratory tomato. The aim of research is prepared mini tomato for
genomic study. The research was conducted at Politeknik Negeri Lampung Tissue Culture
Laboratory from April to September 2020. The material used for this research was mini tomato
seeds. The explants used for the multiplication in this study were the cotyledons. These studies
used macro elements, microelements, tomato vitamins, CaCl2, and FeSO4. EDTA to make
Murashige and Skoog (MS) media, co-cultivation media, and callus induction media, shoot
induction media, and root induction media for generated tomato plants. We successfully
developed a mini tomato until final root induction. The result of these studies will use for
genomic study (next research).