Adaptasi Kecambah Padi Sawah (Oryza sativa L.) Varietas Ciherang dan Ciliwung terhadap Defisit Air yang Diinduksi Dengan Polietilen Glikol 6000
DOI:
https://doi.org/10.25181/prosemnas.v0i0.452Abstrak
The objective of this study was to know whether the seedling of the two varieties of lowlandrice Ciliwung and Ciherang can grow well in condition of water stress induced bypolyethylene glycol 6000. This research was conducted in the Laboratory of Plant Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences,University of Lampung in December 2014, in a 2x3 factorial experiment. Factor A was alowland rice varieties with two levels : Ciherang and Ciliwung. Factor B was thepolyethylene glycol 6000 with 3 levels : 0% w/v, 10% w/v and 20% w/v. Each combinationof treatment was repeated 5 times. The number of experimental unit was 30. The variablein this study were shoot length, fresh weight, dry weight and total chlorophyll content. Datawas analyzed using ANOVA at 5% significance level and proceed with the determination ofa simple effect with the LSD test at 5% significance level. The results showed that Ciheranghave better growth characteristics than Ciliwung. Both varieties negatively responsed topolyethylene glycol 6000 indicated by a decrease in fresh weight, dry weight and total chlorophyll content, while shoots length Ciherang showed a positive response. During seedling growth Ciliwung more adaptive to drought than Ciherang varieties indicated by an increase in the ratio of root shoot and an increased proportion of roots. The final conclusion was that Ciherang more potential to develop in arid region than Ciliwung.Keyword : Lowland rice, Ciherang, Ciliwung, Poliethylen glycol 6000, Shoot length, Freshweight, Dry weight, Chlorophyll content.Unduhan
Referensi
Reni Agustina, Zulkifli, dan Tundjung Tripeni Handayani: Adaptasi Kecambah Padi Sawah.......
Prosiding Seminar Nasional Swasembada Pangan Polinela 29 April 2015 53
Soemarotno, S. 1985. Kajian Gaya Cabut sebagai Metode Penyaringan Ketahanan terhadap Kekeringan dan
Genetika Perakaran Tanaman Padi Lahan Kering. Disertasi Doktor UGM. Yogyakarta. (Tidak
dipublikasikan).
Van der Mescht A., J.A.de Ronde andF.T.Rossouw. 1999. Chlorophyll fluorescence and chlorophyll
contentas a measure of drought tolerance inpotato. South African J. of Science. 95: p. 407-412.
Vergara, B.S. 1995. Bercocok Tanam Padi. Program Nasional PHT Pusat . Departemen Pertanian. Jakarta.
Verslues, P.E., M. Agarwal, K.S.Agarwal, and J. Zhu. 2006. Methods and concepts in quantifying
resistance to drought, salt and freezing, abiotic stress that affect plant water status. Plant J. 45:523-
Yamasaki, S and Dillenburg L.R. 1999. Measurements Of Leaf Relative Water Content In Araucaria
Angustifolia. Revista Brasileira de Fisiologia Vegetal, 11(2):69-75.
Yuliana, N., Ermavitalini, D., Agisimanto D. 2013. Effektivitas meta-Topolin (mT) dan NAA Terhadap
Pertumbuhan In Vitro Stoberi (Fragaria ananassa Var. Dorit) pada Media MS Cair dan Ketahanan
di Media Aklimatisasi. Jurnal Sains dan Seni POMITS Vol 2:2337-3520.Adwitarsa, I. G.B. 1996. Evaluasi Ketahanan terhadap Kekeringan beberapa Varietas Jagung. Tesis Master
Pascasarjana Ilmu-ilmu Pertanian. UGM. Yogyakarta.
Effendi, Y. 2008. Kajian Resistensi Beberapa Varietas Padi Gogo (Oryza sativa L.) Terhadap Cekaman
Kekeringan. Tesis Magister. Universitas sebelas maret press. Surakarta.
Afa La Ode, Purwoko B.S., Junaedi A., Haridjaja O., dan Dewi I.S. 2012. Deteksi Dini Toleransi Padi
Hibrida terhadap Kekeringan menggunakan PEG 6000. J. Agron 41 (1) : 9 - 15 (2013).
Lestari. 2005. Hubungan antara Kerapatan Stomata dengan Ketahanan Kekeringan padaSomaklon Padi
Gajahmungkur, Towuti, dan IR 64. Balai Besar Penelitian dan Pengembangan Bioteknologi dan
Sumberdaya Genetik Pertanian (Balitbiogen), Bogor.
Levitt, J. 1980. Responses of plant to environmental stresses. Volume II. Water, Radiation, Salt, and Other
Stresses. Academic Press. Inc . new York. 607p.
Michel, B.E. and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glicol 6000. Plant Physiol
:914-916.
Miazek, Mgr inz Krystian. 2002. Chlorophill extraction from harvested plant material. Supervisor: Prof. Dr
hab. Inz Stanislaw Ledakowics.
Nio, S.A. dan Y. Banyo. 2011. Konsentrasi Klorofil Daun sebagai Indikator Kekurangan Air pada
Tanaman. J. Ilmiah Sains. 11 ( 2): p. 167-173.
Sani, Daoda Ousmane and Mouhamadou Mounkaila Boureima. 2014. Effect of polyethylene glycol (PEG)
on germination and seedling growth of pearl millet [Pennicetum glaucum (L.) R. Br.] and LD50
for in vitro screening for draught tolerance. African Journal of Biotechnology. Vol. 13 (37), pp
-3747.
Sidari, M., C. Mallamaci, and A. Muscolo. 2008. Drought, salinity and heat differently affect seed
germination of Pinus pinea. J Forest Res 13:326-330.