Kandungan Klorofil Daun Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Seleksi dengan Asam Salisilat secara In vitro

Eka Susilowati, Endang Nurcahyani, Martha Lulus Lande

Sari


Phalaenopsis amabilis is one of Indonesias national flowers. P.amabilis has beautiful
flowers and exciting and high economic value. However, there are some constraints in its development, one of which is Fusarium wilt caused byFusarium oxysporum, for this is not maximized in handling. One of effective procedure to control the disease is to use salicylic acid as a selective agent resistance to the disease. Study of the effect of the addition of salicylic acid(concentrations 15, 30, 45 and 60 ppm) have been studied in vitro in mediumVW(Vacint and Went) on the content ofchlorophyll a, b, and total leaf plantlets P.amabilis. The research was implemented in tissue culture laboratory, Department of Biology, Faculty of MIPA, University of Lampung from February to March 2015. This study used a completely randomized design with 3 replications. Analysis of variance and LSD test performed at 5% significance level. Calculation and extraction of chlorophyll was conducted by Harbourne method(1987). The absorbance was measured with a (Shimadzu UV 800)spectrophotometer at wavelength of 663 and 646 nm. The results showed that the content of chlorophyll a, b, and total plantlets of P. amabilis leaves significantly increased at the concentration of salicylic acid in the medium VW 15, 30, 45 and 60 ppm compared with control(0 ppm).


Key words : Phalaenopsis amabilis plantlets, chlolophyll, salicylic acid, VW medium.


Teks Lengkap:

PDF

Referensi


Anggarwulan. E dan Solochatun. 2007. Kajian Klorofil dan Karotenoid Plantago major L. dan Phaseolus

vulgaris L. sebagai Bioindikator Kualitas Udara. Biodiversitas Volume 8, Nomor 4 Halaman:

-282.

Corina V. A., D. A. Dempsey, and D. F. Klessig. 2009. Salicylic Acid, a Multifaceted Hormone to Combat

Disease. Annu. Rev. Phythopathol 47: 177-206.

Czerpak, R., Dobrzyn,P., Krotke, A., and Kicinska, E. 2002. The Effect of Auxins and salicylic acid on

chlorophyll and Carotenoid Contents in Wolffia Arrhiza (L.) Wimm(Lemnaceae) Growing on

Media of Various Trophicities. Polish Journal of Environmetal Studies.

Djatnika, I. 2012. Seleksi Bakteri Antagonis Untuk Mengendalikan Layu Fusarium pada Tanaman

Phalaenopsis. J. Hort. 22(3):276-284,2012.

Freeman S., A. Zveibel, H. Vintal and M. Maymon. 2002. Isolation of nonpatogenic mutants of Fusarium

oxysporum f.sp. melonis for biological kontrol of Fusarium wilts in cucurbits. Phytopathology

:164-168.

Harbourne JB. 1987. Metode Fitokimia. Terjemahan:Padmawinata K & Sudiro I Penerbit ITB Bandung.

Puspitaningtyas, D.M. dan S. Mursidawati. 2010. Koleksi Anggrek Kebun Raya Bogor. Vol. 1, No. 2

Bogor: UPT Balai Pengembangan Kebun Raya LIPI.

Radwan, D.E.M, and Soltan D.M. 2012. The Negative Effects of Clethodim in Photosyntesis and Gas

Exchange Status of Maize Plants are Ameliorated by Salicylic Acid Pretreatment.

Photosynthetica.

Rebbeca, L., B. Larson, and B.J. Jacobsen. 2007. Biocontrol elicited systemic resistance in sugarbeet is

salicylic acid independent and NPR1 dependent. J. Sugarbeet Res. Vol. 44 Nos. 1&2.

Semangun, H. 1989. Penyakit-Penyakit Tanaman Hortikultura Di Indonesia. Gadjah Mada University

Press. Yogyakarta.




DOI: http://dx.doi.org/10.25181/prosemnas.v0i0.514

Refbacks

  • Saat ini tidak ada refbacks.


Unit Penelitian dan Pengabdian kepada Masyarakat
Politeknik Negeri Lampung
Jln. Soekarno Hatta No. 10 Rajabasa 
Bandar Lampung
Telp/Faks : 0721-703995/0721-787309
Email : semnas@polinela.ac.id
http://semnas.polinela.ac.id
Contact Person
  1. Analianasari (082180229291)
  2. Jakti Kusuma (082216054393) 
  3. Adryade Reshi Gusta (082281898811)