Kandungan Klorofil Daun Planlet Pisang Raja Bulu (Musa paradisiaca L. var sapientum) Hasil Seleksi In vitro terhadap Cekaman Kekeringan

Abstrak

Drought stress which occurs in plants capable of influence plant physiological conditions one of which is chlorophyll. A study of the effect of Polyethylene glycol 6000 with concentration 5%,10%,15% and 20% compared with control (0%) of chlorophyll content a, b, and total leaves of Banana raja bulu plantlets were planted in the solid medium Murashige and Skoog (MS) has been undergone . Research has been carried out in Laboratory of Tissue Culture Departement of Biology FMIPA, University of Lampung from January to March 2015. This study used a completely randomized design with 5 replications. Data were analyzed of variance and LSD test performed at 5% significance level. Extraction and Calculation of chlorophyll was conducted by Harbourne method (1987). The solvent used was 80% acetone. The absorbance was measured with a (Shimudzu UV 800) spectrophotometer at wavelength (λ) of 646 nm and 663 nm. The results showed that the content of chlorophyll a, b, and total plantlets of banana raja bulu leaves significantly decreased at the concentration PEG 6000 of 5%,10%,15% and 20%. This case showed that PEG 6000 capable to lower the water potential in the solid MS medium, so that plant physiological experiencing drought stress result in lower chlorophyll Content of Leaves of Banana raja bulu plantlet.Key words : Drought stress, Chlolophyll, PEG 6000 and Musa paradisiaca L. varsapientum