Kandungan Klorofil Planlet Vanili (Vanilla planifolia Andrews) Hasil Seleksi Ketahanan terhadap Cekaman Kekeringan secara In Vitro

M. Sobran Jamil, Endang Nurcahyani, Zulkifli Zulkifli

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A Study of the content of Chlorophyll a, b and the total on vanilla which was swlwcted in vitro by adding up polyethylene glycol (PEG) 6000 to MS medium at various consentrations of 5%, 10%, 15%, and 20% had been conducted at the Laboratory of Tissue Culture, MIPA faculty, Biology Department, Lampung University, on January to March 2015. At experiment was arranged in a complete random design with six replication. The analysis of variance and BNT test were evaluated in real level of 5%. The calculation and extraction of chlorophyll were conducted by using Harborn (1987) method. This experiment used aceton 80% as solution. The absorbent was measured by using spectrophotometer Shimudzu in 646
and 663 wave length.The result showed that the content of chlorophyll a and b and the total
on vanilla planlet were decreased. The content of Chlorophyll a and total of the MS medium
which were treated by 5% to 20% concentrations of PEG 6000 was decreased compared to
the control. As for the b chlorophyll, only medium with 15% and 20% threatment were
decreased compared to the control.

Key word : Vanilla planifolia Andrews, Chlorophyll, PEG 6000, Drought Stress


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